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RAD23A Antibody

Purified Mouse Monoclonal Antibody

  • E - RAD23A Antibody AO2164a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - RAD23A Antibody AO2164a

    Figure 2:Western blot analysis using RAD23A mAb against human RAD23A (AA: 1-363) recombinant protein. (Expected MW is 42.5 kDa)

  • WB - RAD23A Antibody AO2164a

    Figure 3:Western blot analysis using RAD23A mAb against HEK293 (1) and RAD23A (AA: 1-363)-hIgGFc transfected HEK293 (2) cell lysate.

  • FC - RAD23A Antibody AO2164a

    Figure 4:Flow cytometric analysis of HeLa cells using RAD23A mouse mAb (green) and negative control (red).

  • IHC - RAD23A Antibody AO2164a

    Figure 5:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using RAD23A mouse mAb with DAB staining.

  • IHC - RAD23A Antibody AO2164a

    Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using RAD23A mouse mAb with DAB staining.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P54725
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3E3F4
Isotype IgG2b
Calculated MW 40kDa
Description The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair. Proteins in this family have a modular domain structure consisting of an ubiquitin-like domain (UbL), ubiquitin-associated domain 1 (UbA1), XPC-binding domain and UbA2. The protein encoded by this gene plays an important role in nucleotide excision repair and also in delivery of polyubiquitinated proteins to the proteasome. Alternative splicing results in multiple transcript variants encoding multiple isoforms.
Immunogen Purified recombinant fragment of human RAD23A (AA: 1-363) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 5886
Other Names UV excision repair protein RAD23 homolog A, HR23A, hHR23A, RAD23A
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAD23A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAD23A
Function Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. Involved in vpr-dependent replication of HIV-1 in non- proliferating cells and primary macrophages. Required for the association of HIV-1 vpr with the host proteasome.
Cellular Location Nucleus.
Citations (0)

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1.Biochem Biophys Res Commun. 2013 Feb 22;431(4):686-92.2.Exp Cell Res. 2001 Jul 15;267(2):243-57.

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$ 385.00
Cat# AO2164a
(40 western blots)
Availability: 7-10 days
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