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PRDM5 Antibody

Purified Mouse Monoclonal Antibody

  • E - PRDM5 Antibody AO2166a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - PRDM5 Antibody AO2166a

    Figure 2:Western blot analysis using PRDM5 mAb against human PRDM5 (AA: 1-100) recombinant protein. (Expected MW is 37.6 kDa)

  • WB - PRDM5 Antibody AO2166a

    Figure 3:Western blot analysis using PRDM5 mAb against HEK293 (1) and PRDM5 (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.

  • WB - PRDM5 Antibody AO2166a

    Figure 4:Western blot analysis using PRDM5 mouse mAb against HL-60 (1), NIH/3T3 (2), SW480 (3), and HEK293 (4) cell lysate.

  • IF - PRDM5 Antibody AO2166a

    Figure 5:Immunofluorescence analysis of MCF-7 cells using PRDM5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • FC - PRDM5 Antibody AO2166a

    Figure 6:Flow cytometric analysis of HeLa cells using PRDM5 mouse mAb (green) and negative control (red).

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NQX1
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 7D4C12
Isotype IgG1
Calculated MW 73kDa
Description The protein encoded by this gene is a transcription factor of the PR-domain protein family. It contains a PR-domain and multiple zinc finger motifs. Transcription factors of the PR-domain family are known to be involved in cell differentiation and tumorigenesis.
Immunogen Purified recombinant fragment of human PRDM5 (AA: 1-100) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 11107
Other Names PR domain zinc finger protein 5, 2.1.1.-, PR domain-containing protein 5, PRDM5, PFM2
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/100 - 1/500
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPRDM5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PRDM5
Synonyms PFM2
Function Sequence-specific DNA-binding transcription factor. Represses transcription at least in part by recruitment of the histone methyltransferase EHMT2/G9A and histone deacetylases such as HDAC1. Regulates hematopoiesis-associated protein-coding and microRNA (miRNA) genes. May regulate the expression of proteins involved in extracellular matrix development and maintenance, including fibrillar collagens, such as COL4A1 and COL11A1, connective tissue components, such as HAPLN1, and molecules regulating cell migration and adhesion, including EDIL3 and TGFB2. May caused G2/M arrest and apoptosis in cancer cells.
Cellular Location Nucleus
Tissue Location Widely expressed with highest levels in colon and ovary. Tends to be silenced in breast, colorectal, gastric and liver cancer tissues.
Citations (0)

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1.Tumour Biol. 2014 May;35(5):4509-16.2.J Cancer Res Clin Oncol. 2010 Dec;136(12):1821-5.

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$ 385.00
Cat# AO2166a
(40 western blots)
Availability: 7-10 days
Bulk Size
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