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BMP4 Antibody

Purified Mouse Monoclonal Antibody

  • E - BMP4 Antibody AO2171a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - BMP4 Antibody AO2171a

    Figure 2:Western blot analysis using BMP4 mAb against human BMP4 (AA: 277-408) recombinant protein. (Expected MW is 41 kDa)

  • WB - BMP4 Antibody AO2171a

    Figure 3:Western blot analysis using BMP4 mAb against HEK293 (1) and BMP4 (AA: 277-408)-hIgGFc transfected HEK293 (2) cell lysate.

  • WB - BMP4 Antibody AO2171a

    Figure 4:Western blot analysis using BMP4 mouse mAb against A549 (1), HepG2 (2), and C6 (3) cell lysate.

  • IF - BMP4 Antibody AO2171a

    Figure 5:Immunofluorescence analysis of Hela cells using BMP4 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

  • IF - BMP4 Antibody AO2171a

    Figure 6:Immunofluorescence analysis of Hela cells using BMP4 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • FC - BMP4 Antibody AO2171a

    Figure 7:Flow cytometric analysis of Hela cells using BMP4 mouse mAb (green) and negative control (red).

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P12644
Reactivity Human, Rat
Host Mouse
Clonality Monoclonal
Clone Names 3C11C7
Isotype IgG1
Calculated MW 46.5kDa
Description The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein.
Immunogen Purified recombinant fragment of human BMP4 (AA: 277-408) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 652
Other Names Bone morphogenetic protein 4, BMP-4, Bone morphogenetic protein 2B, BMP-2B, BMP4, BMP2B, DVR4
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/100 - 1/500
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBMP4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BMP4
Synonyms BMP2B, DVR4
Function Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction (By similarity).
Cellular Location Secreted, extracellular space, extracellular matrix
Tissue Location Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines
Research Areas
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1.Cancer Invest. 2013 Oct;31(8):555-62. 2.Eur J Oral Sci. 2013 Aug;121(4):313-8.

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$ 385.00
Cat# AO2171a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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