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FOXM1 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - FOXM1 Antibody AO2180a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

    detail
  • WB - FOXM1 Antibody AO2180a

    Figure 2:Western blot analysis using FOXM1 mAb against human FOXM1 (AA: 649-748) recombinant protein. (Expected MW is 36.6 kDa)

    detail
  • WB - FOXM1 Antibody AO2180a

    Figure 3:Western blot analysis using FOXM1 mAb against HEK293 (1) and FOXM1 (AA: 649-748)-hIgGFc transfected HEK293 (2) cell lysate.

    detail
  • FC - FOXM1 Antibody AO2180a

    Figure 4:Flow cytometric analysis of HeLa cells using FOXM1 mouse mAb (green) and negative control (red).

    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q08050
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6F11A8
Isotype IgG1
Calculated MW 84.3kDa
Description The protein encoded by this gene is a transcriptional activator involved in cell proliferation. The encoded protein is phosphorylated in M phase and regulates the expression of several cell cycle genes, such as cyclin B1 and cyclin D1. Several transcript variants encoding different isoforms have been found for this gene.
Immunogen Purified recombinant fragment of human FOXM1 (AA: 649-748) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 2305
Other Names Forkhead box protein M1, Forkhead-related protein FKHL16, Hepatocyte nuclear factor 3 forkhead homolog 11, HFH-11, HNF-3/fork-head homolog 11, M-phase phosphoprotein 2, MPM-2 reactive phosphoprotein 2, Transcription factor Trident, Winged-helix factor from INS-1 cells, FOXM1, FKHL16, HFH11, MPP2, WIN
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFOXM1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FOXM1
Synonyms FKHL16, HFH11, MPP2, WIN
Function Transcription factor regulating the expression of cell cycle genes essential for DNA replication and mitosis (PubMed:19160488, PubMed:20360045). Plays a role in the control of cell proliferation (PubMed:19160488). Also plays a role in DNA break repair, participating in the DNA damage checkpoint response (PubMed:17101782). Promotes transcription of PHB2 (PubMed:33754036).
Cellular Location Nucleus.
Tissue Location Expressed in thymus, testis, small intestine, colon followed by ovary. Appears to be expressed only in adult organs containing proliferating/cycling cells or in response to growth factors. Also expressed in epithelial cell lines derived from tumors Not expressed in resting cells. Isoform 2 is highly expressed in testis
Research Areas
Citations (0)
citation

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References

1.Cancer Gene Ther. 2014 Mar;21(3):133-8.2.Mol Cancer. 2013 Dec 10;12:159.

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$ 385.00
Cat# AO2180a
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