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CBX5 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - CBX5 Antibody AO2181a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

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  • WB - CBX5 Antibody AO2181a

    Figure 2:Western blot analysis using CBX5 mAb against human CBX5 (AA: 1-191) recombinant protein. (Expected MW is 48.2 kDa)

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  • WB - CBX5 Antibody AO2181a

    Figure 3:Western blot analysis using CBX5 mAb against HEK293 (1) and CBX5 (AA: 1-191)-hIgGFc transfected HEK293 (2) cell lysate.

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  • WB - CBX5 Antibody AO2181a

    Figure 4:Western blot analysis using CBX5 mouse mAb against Hela (1), NIH/3T3 (2), K562 (3), MCF-7 (4), Jurkat (5), and A431 (6) cell lysate.

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  • IF - CBX5 Antibody AO2181a

    Figure 5:Immunofluorescence analysis of Hela cells using CBX5 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

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  • IF - CBX5 Antibody AO2181a

    Figure 6:Immunofluorescence analysis of Hela cells using CBX5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

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  • IHC - CBX5 Antibody AO2181a

    Figure 7:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CBX5 mouse mAb with DAB staining.

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  • IHC - CBX5 Antibody AO2181a

    Figure 8:Immunohistochemical analysis of paraffin-embedded esophagus tissues using CBX5 mouse mAb with DAB staining.

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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession P45973
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 1B10B2
Isotype IgG1
Calculated MW 22.2kDa
Description This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Immunogen Purified recombinant fragment of human CBX5 (AA: 1-191) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 23468
Other Names Chromobox protein homolog 5, Antigen p25, Heterochromatin protein 1 homolog alpha, HP1 alpha, CBX5, HP1A
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCBX5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CBX5
Synonyms HP1A
Function Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Cellular Location Nucleus. Chromosome. Chromosome, centromere Note=Colocalizes with HNRNPU in the nucleus (PubMed:19617346) Component of centromeric and pericentromeric heterochromatin Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase (PubMed:19617346) Localizes to sites of DNA damage (PubMed:28977666)
Research Areas
Citations (0)
citation

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References

1.Mol Carcinog. 2011 Aug;50(8):601-13.2.J Mol Biol. 2013 Jan 9;425(1):54-70.

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$ 385.00
Cat# AO2181a
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