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ATG13 Antibody

Purified Mouse Monoclonal Antibody

  • E - ATG13 Antibody AO2184a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - ATG13 Antibody AO2184a

    Figure 2:Western blot analysis using ATG13 mAb against human ATG13 (AA: 339-550) recombinant protein. (Expected MW is 48.9 kDa)

  • WB - ATG13 Antibody AO2184a

    Figure 3:Western blot analysis using ATG13 mAb against HEK293 (1) and ATG13 (AA: 339-550)-hIgGFc transfected HEK293 (2) cell lysate.

  • IHC - ATG13 Antibody AO2184a

    Figure 4:Immunohistochemical analysis of paraffin-embedded striated muscle tissues using ATG13 mouse mAb with DAB staining.

  • IHC - ATG13 Antibody AO2184a

    Figure 5:Immunohistochemical analysis of paraffin-embedded cardiac muscle tissues using ATG13 mouse mAb with DAB staining.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O75143
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6B2E12
Isotype IgG1
Calculated MW 56.6kDa
Description ATG13 (Autophagy Related 13) is a Protein Coding gene. Among its related pathways are Senescence and Autophagy and mTOR signaling pathway. GO annotations related to this gene include protein kinase binding.
Immunogen Purified recombinant fragment of human ATG13 (AA: 339-550) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 9776
Other Names Autophagy-related protein 13, ATG13, KIAA0652
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATG13 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATG13
Synonyms KIAA0652
Function Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1- RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation.
Cellular Location Cytoplasm, cytosol. Preautophagosomal structure. Note=Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane; the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation.
Research Areas
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1.Mol Biol Cell. 2009 Apr;20(7):1992-2003. 2.Mol Cell Biol. 2009 Jan;29(1):157-71.

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$ 385.00
Cat# AO2184a
(40 western blots)
Availability: 7-10 days
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