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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ATG14L Antibody   

ATG14L Antibody

Purified Mouse Monoclonal Antibody

  • E - ATG14L Antibody AO2189a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - ATG14L Antibody AO2189a

    Figure 2:Western blot analysis using ATG14L mAb against human ATG14L (AA: 43-303) recombinant protein. (Expected MW is 56.4 kDa)

  • WB - ATG14L Antibody AO2189a

    Figure 3:Western blot analysis using ATG14L mAb against HEK293 (1) and ATG14L (AA: 43-303)-hIgGFc transfected HEK293 (2) cell lysate.

  • IHC - ATG14L Antibody AO2189a

    Figure 4:Immunohistochemical analysis of paraffin-embedded testis tissues using ATG14L mouse mAb with DAB staining.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q6ZNE5
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1F7C10
Isotype IgG1
Calculated MW 55.3kDa
Description ATG14 (Autophagy Related 14) is a Protein Coding gene. Diseases associated with ATG14 include human granulocytic anaplasmosis. Among its related pathways are Senescence and Autophagy and Regulation of autophagy.
Immunogen Purified recombinant fragment of human ATG14L (AA: 43-303) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 22863
Other Names Beclin 1-associated autophagy-related key regulator, Barkor, Autophagy-related protein 14-like protein, Atg14L, ATG14, KIAA0831
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATG14L Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATG14
Synonyms KIAA0831
Function Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1 (PubMed:23878393). Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK (By similarity).
Cellular Location Cytoplasm. Endoplasmic reticulum membrane; Peripheral membrane protein. Preautophagosomal structure membrane; Peripheral membrane protein. Note=Cytosolic under nutrient-rich conditions. Following autophagy stimuli, such as starvation or rapamycin induction, predominantly detected in cytoplasmic foci, identified as isolation membranes and autophagosomes. Accumulates on highly curved PtdIns(3)P enriched autophagic membrane via its BATS domain to sense and maintain membrane curvature (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (By similarity)
Research Areas
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1.Mol Cell Biol. 2013 Sep;33(18):3675-88. 2.J Biol Chem. 2012 Nov 9;287(46):39107-14.

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$ 385.00
Cat# AO2189a
(40 western blots)
Availability: 7-10 days
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