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KDM6A Antibody

Purified Mouse Monoclonal Antibody

  • E - KDM6A Antibody AO2213a

    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • WB - KDM6A Antibody AO2213a

    Figure 2:Western blot analysis using KDM6A mAb against human KDM6A (AA: 1252-1401) recombinant protein. (Expected MW is 43.4 kDa)

  • WB - KDM6A Antibody AO2213a

    Figure 3:Western blot analysis using KDM6A mAb against HEK293 (1) and KDM6A (AA: 1252-1401)-hIgGFc transfected HEK293 (2) cell lysate.

  • FC - KDM6A Antibody AO2213a

    Figure 4:Flow cytometric analysis of Hela cells using KDM6A mouse mAb (green) and negative control (red).

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O15550
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5C2A9
Isotype IgG2a
Calculated MW 154kDa
Description This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3. Multiple alternatively spliced transcript variants have been found for this gene.
Immunogen Purified recombinant fragment of human KDM6A (AA: 1252-1401) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 7403
Other Names Lysine-specific demethylase 6A, 1.14.11.-, Histone demethylase UTX, Ubiquitously-transcribed TPR protein on the X chromosome, Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein, KDM6A, UTX
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKDM6A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KDM6A
Synonyms UTX
Function Histone demethylase that specifically demethylates 'Lys- 27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Demethylation of 'Lys-27' of histone H3 is concomitant with methylation of 'Lys-4' of histone H3, and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A.
Cellular Location Nucleus.
Citations (0)

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1.Stem Cells. 2014 Mar;32(3):802-15. 2.Proc Natl Acad Sci U S A. 2011 Feb 1;108(5):2130-5.

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$ 385.00
Cat# AO2213a
(40 western blots)
Availability: 7-10 days
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