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PLCG1 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - PLCG1 Antibody AO2220a

    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

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  • WB - PLCG1 Antibody AO2220a

    Figure 2:Western blot analysis using PLCG1 mAb against human PLCG1 (AA: 1192-1291) recombinant protein. (Expected MW is 37.5 kDa)

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  • WB - PLCG1 Antibody AO2220a

    Figure 3:Western blot analysis using PLCG1 mAb against HEK293 (1) and PLCG1 (AA: 1192-1291)-hIgGFc transfected HEK293 (2) cell lysate.

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  • WB - PLCG1 Antibody AO2220a

    Figure 4:Western blot analysis using PLCG1 mouse mAb against NIH/3T3 (1), Jurkat (2), A431 (3), and Hela (4) cell lysate.

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  • IF - PLCG1 Antibody AO2220a

    Figure 5:Immunofluorescence analysis of Hela cells using PLCG1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

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  • FC - PLCG1 Antibody AO2220a

    Figure 6:Flow cytometric analysis of Raji cells using PLCG1 mouse mAb (green) and negative control (red).

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  • IHC - PLCG1 Antibody AO2220a

    Figure 7:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using PLCG1 mouse mAb with DAB staining.

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  • IHC - PLCG1 Antibody AO2220a

    Figure 8:Immunohistochemical analysis of paraffin-embedded esophagus tissues using PLCG1 mouse mAb with DAB staining.

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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, ICC, E
Primary Accession P19174
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 3H1C10
Isotype IgG1
Calculated MW 148.5kDa
Description The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.
Immunogen Purified recombinant fragment of human PLCG1 (AA: 1192-1291) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 5335
Other Names 1-phosphatidylinositol 4, 5-bisphosphate phosphodiesterase gamma-1, 3.1.4.11, PLC-148, Phosphoinositide phospholipase C-gamma-1, Phospholipase C-II, PLC-II, Phospholipase C-gamma-1, PLC-gamma-1, PLCG1, PLC1
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPLCG1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PLCG1 (HGNC:9065)
Synonyms PLC1
Function Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, EGFR, FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Plays a role in actin reorganization and cell migration (PubMed:17229814). Guanine nucleotide exchange factor that binds the GTPase DNM1 and catalyzes the dissociation of GDP, allowing a GTP molecule to bind in its place, therefore enhancing DNM1-dependent endocytosis (By similarity).
Cellular Location Cell projection, lamellipodium. Cell projection, ruffle. Note=Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment.
Research Areas
Citations (0)
citation

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References

1.Cancer Discov. 2014 Apr;4(4):OF13.2.Int J Cancer. 2013 Mar 1;132(5):1022-31.

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$ 385.00
Cat# AO2220a
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