|Application ||WB, IHC, FC, ICC, E|
|Description||This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X.|
|Immunogen||Purified recombinant fragment of human RF1 (AA: 288-437) expressed in E. Coli.|
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Other Names||Eukaryotic peptide chain release factor subunit 1, Eukaryotic release factor 1, eRF1, Protein Cl1, TB3-1, ETF1, ERF1, RF1, SUP45L1|
WB~~1/200 - 1/1000
FC~~1/200 - 1/400
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ERF1, RF1, SUP45L1|
|Function||Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.|
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1.Nucleic Acids Res. 2013 Apr;41(8):4573-86. 2.Protein Sci. 2012 Jun;21(6):896-903.
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