OTUD4 Antibody
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, FC, E |
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Primary Accession | Q01804 |
Reactivity | Human, Rat |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 3B12G11 |
Isotype | IgG1 |
Calculated MW | 124kDa |
Description | Alternatively spliced transcript variants have been found for this gene. The smaller protein isoform encoded by the shorter transcript variant is found only in HIV-1 infected cells. |
Immunogen | Purified recombinant fragment of human OTUD4 (AA: 815-1049) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Gene ID | 54726 |
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Other Names | OTU domain-containing protein 4, 3.4.19.12, HIV-1-induced protein HIN-1, OTUD4, HIN1, KIAA1046 |
Dilution | E~~1/10000 WB~~1/500 - 1/2000 FC~~1/200 - 1/400 |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | OTUD4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | OTUD4 (HGNC:24949) |
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Function | Deubiquitinase which hydrolyzes the isopeptide bond between the ubiquitin C-terminus and the lysine epsilon-amino group of the target protein (PubMed:23827681, PubMed:25944111, PubMed:29395066). May negatively regulate inflammatory and pathogen recognition signaling in innate immune response. Upon phosphorylation at Ser-202 and Ser-204 residues, via IL-1 receptor and Toll-like receptor signaling pathway, specifically deubiquitinates 'Lys-63'-polyubiquitinated MYD88 adapter protein triggering down-regulation of NF-kappa-B-dependent transcription of inflammatory mediators (PubMed:29395066). Independently of the catalytic activity, acts as a scaffold for alternative deubiquitinases to assemble specific deubiquitinase- substrate complexes. Associates with USP7 and USP9X deubiquitinases to stabilize alkylation repair enzyme ALKBH3, thereby promoting the repair of alkylated DNA lesions (PubMed:25944111). |
Cellular Location | Cytoplasm. Nucleus. Note=Primarily cytoplasmic |
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Provided below are standard protocols that you may find useful for product applications.
References
1.EMBO J. 2015 Jun 12;34(12):1687-703.
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