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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Rab1b Antibody   

Rab1b Antibody

Purified Mouse Monoclonal Antibody

  • E - Rab1b Antibody AO2225a

    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • WB - Rab1b Antibody AO2225a

    Figure 2:Western blot analysis using Rab1b mAb against human Rab1b (AA: 60-201) recombinant protein. (Expected MW is 41.4 kDa)

  • WB - Rab1b Antibody AO2225a

    Figure 3:Western blot analysis using Rab1b mAb against HEK293 (1) and Rab1b (AA: 60-201)-hIgGFc transfected HEK293 (2) cell lysate.

  • FC - Rab1b Antibody AO2225a

    Figure 4:Flow cytometric analysis of Hela cells using Rab1b mouse mAb (green) and negative control (red).

  • IHC - Rab1b Antibody AO2225a

    Figure 5:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using Rab1b mouse mAb with DAB staining.

  • IHC - Rab1b Antibody AO2225a

    Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using Rab1b mouse mAb with DAB staining.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9H0U4
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7A12G2
Isotype IgG2b
Calculated MW 22.2kDa
Description Members of the RAB protein family, such as RAB1B, are low molecular mass monomeric GTPases localized on the cytoplasmic surfaces of distinct membrane-bound organelles. RAB1B functions in the early secretory pathway and is essential for vesicle transport between the endoplasmic reticulum (ER) and Golgi
Immunogen Purified recombinant fragment of human Rab1b (AA: 60-201) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 81876
Other Names Ras-related protein Rab-1B, RAB1B
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRab1b Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAB1B
Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB1B regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. Plays a role in the initial events of the autophagic vacuole development which take place at specialized regions of the endoplasmic reticulum.
Cellular Location Cytoplasm. Membrane; Lipid-anchor; Cytoplasmic side. Preautophagosomal structure membrane; Lipid-anchor; Cytoplasmic side. Note=Targeted by REP1 to membranes of specific subcellular compartments including endoplasmic reticulum, Golgi apparatus, and intermediate vesicles between these two compartments. In the GDP-form, colocalizes with GDI in the cytoplasm (By similarity).
Research Areas
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1.Mol Biol Cell. 2013 Mar;24(5):617-32.2.Eur J Cell Biol. 2011 Apr;90(4):301-11.

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$ 385.00
Cat# AO2225a
(40 western blots)
Availability: 7-10 days
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