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VEGFA Antibody

Purified Mouse Monoclonal Antibody

  • E - VEGFA Antibody AO2227a

    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • WB - VEGFA Antibody AO2227a

    Figure 2:Western blot analysis using VEGFA mAb against human VEGFA (AA: 207-371) recombinant protein. (Expected MW is 20.6 kDa)

  • WB - VEGFA Antibody AO2227a

    Figure 3:Western blot analysis using VEGFA mAb against HEK293 (1) and VEGFA (AA: 207-371)-hIgGFc transfected HEK293 (2) cell lysate.

  • WB - VEGFA Antibody AO2227a

    Figure 4:Western blot analysis using VEGFA mouse mAb against HUVEC (1), HEK293 (2), Jurkat (3), and Hela (4) cell lysate.

  • FC - VEGFA Antibody AO2227a

    Figure 5:Flow cytometric analysis of Hela cells using VEGFA mouse mAb (green) and negative control (red).

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P15692
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6G5A10
Isotype IgG1
Calculated MW 27kDa
Description This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site.
Immunogen Purified recombinant fragment of human VEGFA (AA: 207-371) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 7422
Other Names Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGFA, VEGF
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVEGFA Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms VEGF
Function Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
Cellular Location Secreted Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin
Tissue Location Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. A higher level expression seen in pituitary tumors as compared to the pituitary gland
Research Areas
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1.Oncol Rep. 2014 Dec;32(6):2359-64. 2.Eur J Cancer. 2014 Nov;50(16):2855-65.

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$ 385.00
Cat# AO2227a
(40 western blots)
Availability: 7-10 days
Bulk Size
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