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PRDM14 Antibody

Purified Mouse Monoclonal Antibody

  • E - PRDM14 Antibody AO2229a

    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • WB - PRDM14 Antibody AO2229a

    Figure 2:Western blot analysis using PRDM14 mAb against human PRDM14 (AA: 4-203) recombinant protein. (Expected MW is 50.3 kDa)

  • WB - PRDM14 Antibody AO2229a

    Figure 3:Western blot analysis using PRDM14 mAb against HEK293 (1) and PRDM14 (AA: 4-203)-hIgGFc transfected HEK293 (2) cell lysate.

  • FC - PRDM14 Antibody AO2229a

    Figure 4:Flow cytometric analysis of Hela cells using PRDM14 mouse mAb (green) and negative control (red).

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9GZV8
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6H9F1
Isotype IgG1
Calculated MW 64kDa
Description This gene encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer.
Immunogen Purified recombinant fragment of human PRDM14 (AA: 4-203) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 63978
Other Names PR domain zinc finger protein 14, 2.1.1.-, PR domain-containing protein 14, PRDM14
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPRDM14 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PRDM14
Function Transcription factor that has both positive and negative roles on transcription. Required for the maintenance of emryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression (By similarity). Directly up-regulates the expression of pluripotency gene POU5F1 through its proximal enhancer. Binds to the DNA consensus sequence 5'-GGTC[TC]CTAA-3'.
Cellular Location Nucleus.
Tissue Location Expressed in embryonic stem cells. Tends to be overexpressed in breast cancer (at protein level)
Citations (0)

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1.Carcinogenesis. 2014 Nov;35(11):2611-8.2.Med Oncol. 2013;30(3):605.

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$ 385.00
Cat# AO2229a
(40 western blots)
Availability: 7-10 days
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