|Application ||WB, FC, E|
|Description||This gene encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer.|
|Immunogen||Purified recombinant fragment of human PRDM14 (AA: 4-203) expressed in E. Coli.|
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Other Names||PR domain zinc finger protein 14, 2.1.1.-, PR domain-containing protein 14, PRDM14|
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PRDM14 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription factor that has both positive and negative roles on transcription. Required for the maintenance of emryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression. Its association with CBFA2T2 is required for the functions in pluripotency and germ cell formation (By similarity). Directly up-regulates the expression of pluripotency gene POU5F1 through its proximal enhancer. Binds to the DNA consensus sequence 5'-GGTC[TC]CTAA-3'.|
|Tissue Location||Expressed in embryonic stem cells. Tends to be overexpressed in breast cancer (at protein level)|
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1.Carcinogenesis. 2014 Nov;35(11):2611-8.2.Med Oncol. 2013;30(3):605.
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