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RAD50 Antibody

Purified Mouse Monoclonal Antibody

  • E - RAD50 Antibody AO2233a

    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • WB - RAD50 Antibody AO2233a

    Figure 2:Western blot analysis using RAD50 mAb against human RAD50 (AA: 228-359) recombinant protein. (Expected MW is 41.7 kDa)

  • WB - RAD50 Antibody AO2233a

    Figure 3:Western blot analysis using RAD50 mAb against HEK293 (1) and RAD50 (AA: 228-359)-hIgGFc transfected HEK293 (2) cell lysate.

  • WB - RAD50 Antibody AO2233a

    Figure 4:Western blot analysis using RAD50 mouse mAb against C6 (1) and HepG2 (2) cell lysate.

  • FC - RAD50 Antibody AO2233a

    Figure 5:Flow cytometric analysis of Hela cells using RAD50 mouse mAb (green) and negative control (red).

  • IHC - RAD50 Antibody AO2233a

    Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using RAD50 mouse mAb with DAB staining.

  • IHC - RAD50 Antibody AO2233a

    Figure 7:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using RAD50 mouse mAb with DAB staining.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q92878
Reactivity Human, Rat
Host Mouse
Clonality Monoclonal
Clone Names 3D2G10
Isotype IgG2a
Calculated MW 154kDa
Description The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.
Immunogen Purified recombinant fragment of human RAD50 (AA: 228-359) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 10111
Other Names DNA repair protein RAD50, hRAD50, 3.6.-.-, RAD50
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAD50 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAD50
Function Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand- specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
Cellular Location Nucleus. Chromosome, telomere. Note=Localizes to discrete nuclear foci after treatment with genotoxic agents
Tissue Location Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.
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1.Breast Cancer Res Treat. 2010 Sep;123(2):607-9.2.Dis Markers. 2008;24(2):127-34.

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$ 385.00
Cat# AO2233a
(40 western blots)
Availability: 7-10 days
Bulk Size
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