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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   MAP2K3 Antibody   

MAP2K3 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - MAP2K3 Antibody AO2234a

    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • WB - MAP2K3 Antibody AO2234a

    Figure 2:Western blot analysis using MAP2K3 mAb against human MAP2K3 (AA: 1-138) recombinant protein. (Expected MW is 42.1 kDa)

  • WB - MAP2K3 Antibody AO2234a

    Figure 3:Western blot analysis using MAP2K3 mAb against HEK293 (1) and MAP2K3 (AA: 1-138)-hIgGFc transfected HEK293 (2) cell lysate.

  • IF - MAP2K3 Antibody AO2234a

    Figure 4:Immunofluorescence analysis of GC-7901 cells using MAP2K3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • IF - MAP2K3 Antibody AO2234a

    Figure 5:Immunofluorescence analysis of Hela cells using MAP2K3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • IF - MAP2K3 Antibody AO2234a

    Figure 6:Immunofluorescence analysis of HepG2 cells using MAP2K3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • FC - MAP2K3 Antibody AO2234a

    Figure 7:Flow cytometric analysis of Hela cells using MAP2K3 mouse mAb (green) and negative control (red).

  • IHC - MAP2K3 Antibody AO2234a

    Figure 8:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using MAP2K3 mouse mAb with DAB staining.

  • IHC - MAP2K3 Antibody AO2234a

    Figure 9:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MAP2K3 mouse mAb with DAB staining.

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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, ICC, E
Primary Accession P46734
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2E12D11
Isotype IgG1
Calculated MW 39.3kDa
Description The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene.
Immunogen Purified recombinant fragment of human MAP2K3 (AA: 1-138) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 5606
Other Names Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, 2.7.12.2, MAPK/ERK kinase 3, MEK 3, Stress-activated protein kinase kinase 2, SAPK kinase 2, SAPKK-2, SAPKK2, MAP2K3, MEK3, MKK3, PRKMK3, SKK2
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMAP2K3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAP2K3
Synonyms MEK3, MKK3, PRKMK3, SKK2
Function Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
Tissue Location Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues
Research Areas
Citations (0)

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References

1.Hum Mol Genet. 2013 Nov 1;22(21):4438-49.2.Proteomics Clin Appl. 2010 Nov;4(10-11):816-28.

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$ 385.00
Cat# AO2234a
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