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Tlr2 Antibody

Purified Mouse Monoclonal Antibody

  • E - Tlr2 Antibody AO2241a

    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • WB - Tlr2 Antibody AO2241a

    Figure 2:Western blot analysis using Tlr2 mAb against mouse Tlr2 (AA: 628-777) recombinant protein. (Expected MW is 44 kDa)

  • WB - Tlr2 Antibody AO2241a

    Figure 3:Western blot analysis using Tlr2 mAb against HEK293 (1) and Tlr2 (AA: 628-777)-hIgGFc transfected HEK293 (2) cell lysate.

  • IF - Tlr2 Antibody AO2241a

    Figure 4:Immunofluorescence analysis of HepG2 cells using Tlr2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9QUN7
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7G5F4
Isotype IgG1
Calculated MW 90kDa
Description the activation of toll-like receptor (TLR) 2 induces oxidative stress and inflammation, TLR2 may be directly linked to skeletal muscle atrophy.
Immunogen Purified recombinant fragment of mouse Tlr2 (AA: 628-777) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 24088
Other Names Toll-like receptor 2, CD282, Tlr2
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTlr2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Tlr2
Function Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins (By similarity).
Cellular Location Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, phagosome membrane; Single-pass type I membrane protein
Tissue Location Detected in a macrophage cell line, smooth muscle, lung, spleen, thymus, brain and adipose tissue
Research Areas
Citations ( 0 )


1.Biochem Biophys Res Commun. 2015 Apr 10;459(3):534-40.2.Indian J Exp Biol. 2015 Feb;53(2):82-92.

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$ 385.00
Cat# AO2241a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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