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DDX5 Antibody

Purified Mouse Monoclonal Antibody

  • E - DDX5 Antibody AO2243a

    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • WB - DDX5 Antibody AO2243a

    Figure 2:Western blot analysis using DDX5 mAb against human DDX5 (AA: 475-614) recombinant protein. (Expected MW is 41.5 kDa)

  • WB - DDX5 Antibody AO2243a

    Figure 3:Western blot analysis using DDX5 mAb against HEK293 (1) and DDX5 (AA: 475-614)-hIgGFc transfected HEK293 (2) cell lysate.

  • WB - DDX5 Antibody AO2243a

    Figure 4:Western blot analysis using DDX5 mouse mAb against HT-29 (1), Hela (2), NIH/3T3 (3), COS7 (4), SW620 (5), Jurkat (6), A431 (7), and MCF-7 (8) cell lysate.

  • FC - DDX5 Antibody AO2243a

    Figure 5:Flow cytometric analysis of Hela cells using DDX5 mouse mAb (green) and negative control (red).

  • IHC - DDX5 Antibody AO2243a

    Figure 6:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using DDX5 mouse mAb with DAB staining.

  • IHC - DDX5 Antibody AO2243a

    Figure 7:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using DDX5 mouse mAb with DAB staining.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P17844
Reactivity Human, Mouse, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 4F1C12
Isotype IgG2a
Calculated MW 69.1kDa
Description DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified.
Immunogen Purified recombinant fragment of human DDX5 (AA: 475-614) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 1655
Other Names Probable ATP-dependent RNA helicase DDX5,, DEAD box protein 5, RNA helicase p68, DDX5, G17P1, HELR, HLR1
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDDX5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DDX5
Synonyms G17P1, HELR, HLR1
Function Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for estrogen receptor ESR1 and androgen receptor AR. Increases ESR1 AF-1 domain-mediated transactivation and ESR1 AF-1 and AF-2 domains transcriptional synergistic activity. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specicic manner; the function probbaly involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms.
Cellular Location Nucleus, nucleolus
Citations (0)

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1.Cancer Discov. 2012 Sep;2(9):812-25.2.Nucleic Acids Res. 2012 Apr;40(7):3159-71.

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$ 385.00
Cat# AO2243a
(40 western blots)
Availability: 7-10 days
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