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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   SLC2A4 Antibody   

SLC2A4 Antibody

Purified Mouse Monoclonal Antibody

  • E - SLC2A4 Antibody AO2244a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - SLC2A4 Antibody AO2244a
    Figure 1: Western blot analysis using SLC2A4 mAb against human SLC2A4 recombinant protein. (Expected MW is 39.9 kDa)
  • WB - SLC2A4 Antibody AO2244a
    Figure 2: Western blot analysis using SLC2A4 mAb against HEK293 (1) and SLC2A4 (AA: 224-353)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - SLC2A4 Antibody AO2244a
    Figure 3: Western blot analysis using SLC2A4 mouse mAb against NIH3T3 (1), 3T3L1 (2), MCF-7 (4) cell lysate and Mouse heart (3) tissue lysate.
  • IF - SLC2A4 Antibody AO2244a
    Figure 4: Immunofluorescence analysis of HeLa cells using SLC2A4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • IF - SLC2A4 Antibody AO2244a
    Figure 5: Immunofluorescence analysis of HepG2 cells using SLC2A4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • FC - SLC2A4 Antibody AO2244a
    Figure 6: Flow cytometric analysis of HeLa cells using SLC2A4 mouse mAb (green) and negative control (purple).
  • IHC - SLC2A4 Antibody AO2244a
    Figure 7: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using SLC2A4 mouse mAb with DAB staining.
  • IHC - SLC2A4 Antibody AO2244a
    Figure 8: Immunohistochemical analysis of paraffin-embedded cardiac muscle tissues using SLC2A4 mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P14672
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 3G10A3
Isotype IgG2b
Calculated MW 54.8kDa
Description This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM).
Immunogen Purified recombinant fragment of human SLC2A4 (AA: 224-353 ) expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 6517
Other Names Solute carrier family 2, facilitated glucose transporter member 4, Glucose transporter type 4, insulin-responsive, GLUT-4, SLC2A4, GLUT4
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSLC2A4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SLC2A4
Synonyms GLUT4
Function Insulin-regulated facilitative glucose transporter.
Cellular Location Cell membrane; Multi-pass membrane protein. Endomembrane system; Multi-pass membrane protein. Cytoplasm, perinuclear region. Note=Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration
Tissue Location Skeletal and cardiac muscles; brown and white fat
Research Areas
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1.J Biol Chem. 2011 May 13;286(19):16541-5. 2.PLoS One. 2010 Dec 20;5(12):e15560.

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$ 385.00
Cat# AO2244a
(40 western blots)
Availability: 7-10 days
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