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ID2 Antibody

Purified Mouse Monoclonal Antibody

  • E - ID2 Antibody AO2255a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - ID2 Antibody AO2255a
    Figure 1: Western blot analysis using ID2 mAb against human ID2 recombinant protein. (Expected MW is 17.3 kDa)
  • FC - ID2 Antibody AO2255a
    Figure 2: Flow cytometric analysis of SK-N-SH cells using ID2 mouse mAb (green) and negative control (purple).
  • IHC - ID2 Antibody AO2255a
    Figure 3: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ID2 mouse mAb with DAB staining.
  • IHC - ID2 Antibody AO2255a
    Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ID2 mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q02363
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 4E12G5
Isotype IgG1
Calculated MW 15kDa
Description The protein encoded by this gene belongs to the inhibitor of DNA binding family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the inhibitor of DNA binding family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene of this gene is located on chromosome 3.
Immunogen Purified recombinant fragment of human ID2 (AA: 1-134) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 3398
Other Names DNA-binding protein inhibitor ID-2, Class B basic helix-loop-helix protein 26, bHLHb26, Inhibitor of DNA binding 2, Inhibitor of differentiation 2, ID2, BHLHB26
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsID2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ID2
Synonyms BHLHB26
Function Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Restricts the CLOCK and ARNTL/BMAL1 localization to the cytoplasm. Plays a role in both the input and output pathways of the circadian clock: in the input component, is involved in modulating the magnitude of photic entrainment and in the output component, contributes to the regulation of a variety of liver clock-controlled genes involved in lipid metabolism.
Cellular Location Cytoplasm. Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981, ECO:0000269|PubMed:20861012}
Tissue Location Highly expressed in early fetal tissues, including those of the central nervous system
Research Areas
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1.J Neurosci Res. 2012 May;90(5):925-32. 2.Mol Cancer. 2010 Jun 17;9:151.

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$ 385.00
Cat# AO2255a
(40 western blots)
Availability: 7-10 days
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