ID2 Antibody
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, FC, E |
---|---|
Primary Accession | Q02363 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 4E12G5 |
Isotype | IgG1 |
Calculated MW | 15kDa |
Description | The protein encoded by this gene belongs to the inhibitor of DNA binding family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the inhibitor of DNA binding family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene of this gene is located on chromosome 3. |
Immunogen | Purified recombinant fragment of human ID2 (AA: 1-134) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Gene ID | 3398 |
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Other Names | DNA-binding protein inhibitor ID-2, Class B basic helix-loop-helix protein 26, bHLHb26, Inhibitor of DNA binding 2, Inhibitor of differentiation 2, ID2, BHLHB26 |
Dilution | E~~1/10000 WB~~1/500 - 1/2000 FC~~1/200 - 1/400 IHC~~1/200 - 1/1000 |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | ID2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ID2 |
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Synonyms | BHLHB26 |
Function | Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer. Restricts the CLOCK and BMAL1 localization to the cytoplasm. Plays a role in both the input and output pathways of the circadian clock: in the input component, is involved in modulating the magnitude of photic entrainment and in the output component, contributes to the regulation of a variety of liver clock-controlled genes involved in lipid metabolism. |
Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:P41136}. Nucleus {ECO:0000250|UniProtKB:P41136} |
Tissue Location | Highly expressed in early fetal tissues, including those of the central nervous system |
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Provided below are standard protocols that you may find useful for product applications.
References
1.J Neurosci Res. 2012 May;90(5):925-32. 2.Mol Cancer. 2010 Jun 17;9:151.
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