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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   Neurod Antibody   

Neurod Antibody

Purified Mouse Monoclonal Antibody

  • E - Neurod Antibody AO2258a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - Neurod Antibody AO2258a
    Figure 1: Western blot analysis using NEUROD1 mAb against human NEUROD1 recombinant protein. (Expected MW is 33.2 kDa)
  • WB - Neurod Antibody AO2258a
    Figure 2: Western blot analysis using NEUROD1 mAb against HEK293 (1) and NEUROD1 (AA: 26-91)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - Neurod Antibody AO2258a
    Figure 3: Western blot analysis using NEUROD1 mouse mAb against NIH3T3 (1), SK-N-SH (2), COS7 (3), and MCF-7 (4) cell lysate.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13562
Reactivity Human, Mouse, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 6E9G12
Isotype IgG1
Calculated MW 40kDa
Description This gene encodes a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and mutations in this gene result in type II diabetes mellitus.
Immunogen Purified recombinant fragment of human Neurod (AA: 26-91 ) expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 4760
Other Names Neurogenic differentiation factor 1, NeuroD, NeuroD1, Class A basic helix-loop-helix protein 3, bHLHa3, NEUROD1, BHLHA3, NEUROD
Dilution E~~1/10000
WB~~1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNeurod Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Acts as a transcriptional activator: mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. Associates with the p300/CBP transcription coactivator complex to stimulate transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor CDKN1A. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification. Also required for dendrite morphogenesis and maintenance in the cerebellar cortex. Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis (By similarity).
Cellular Location Cytoplasm. Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981, ECO:0000269|PubMed:14752053}. Note=In pancreatic islet cells, shuttles to the nucleus in response to glucose stimulation (By similarity). Colocalizes with NR0B2 in the nucleus.
Research Areas
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1.Cancer Res. 2011 Apr 15;71(8):2938-48. 2.Transplant Proc. 2010 Jul-Aug;42(6):2071-4.

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$ 385.00
Cat# AO2258a
(40 western blots)
Availability: 7-10 days
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