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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   SLC27A5 Antibody   

SLC27A5 Antibody

Purified Mouse Monoclonal Antibody

  • E - SLC27A5 Antibody AO2265a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - SLC27A5 Antibody AO2265a
    Figure 1: Western blot analysis using SLC27A5 mAb against human SLC27A5 recombinant protein. (Expected MW is 32.9 kDa)
  • WB - SLC27A5 Antibody AO2265a
    Figure 2: Western blot analysis using SLC27A5 mAb against HEK293 (1) and SLC27A5 (AA: 508-570)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - SLC27A5 Antibody AO2265a
    Figure 3: Western blot analysis using SLC27A5 mouse mAb against 3T3L1 (1), HepG2 (2), NIH3T3 (3), and PC-3 (4) cell lysate.
  • IHC - SLC27A5 Antibody AO2265a
    Figure 4: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using SLC27A5 mouse mAb with DAB staining.
  • IHC - SLC27A5 Antibody AO2265a
    Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using SLC27A5 mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y2P5
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 9C4D1
Isotype IgG1
Calculated MW 75.4kDa
Description The protein encoded by this gene is an isozyme of very long-chain acyl-CoA synthetase (VLCS). It is capable of activating very long-chain fatty-acids containing 24- and 26-carbons. It is expressed in liver and associated with endoplasmic reticulum but not with peroxisomes. Its primary role is in fatty acid elongation or complex lipid synthesis rather than in degradation. This gene has a mouse ortholog.
Immunogen Purified recombinant fragment of human SLC27A5 (AA: 508-570) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 10998
Other Names Bile acyl-CoA synthetase, BACS,, Bile acid-CoA ligase, BA-CoA ligase, BAL, Cholate--CoA ligase, Fatty acid transport protein 5, FATP-5, Fatty-acid-coenzyme A ligase, very long-chain 3, Solute carrier family 27 member 5, Very long-chain acyl-CoA synthetase homolog 2, VLCS-H2, VLCSH2, Very long-chain acyl-CoA synthetase-related protein, VLACS-related, VLACSR, SLC27A5, ACSB, ACSVL6, FACVL3, FATP5
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSLC27A5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SLC27A5
Function Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation. In vitro, also activates 3-alpha,7- alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol.
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein
Tissue Location Predominantly expressed in liver.
Research Areas
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1.Horm Metab Res. 2010 Nov;42(12):854-9. 2.Mol Nutr Food Res. 2007 Feb;51(2):185-91.

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$ 385.00
Cat# AO2265a
(40 western blots)
Availability: 7-10 days
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