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GNL3 Antibody

Purified Mouse Monoclonal Antibody

  • E - GNL3 Antibody AO2268a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - GNL3 Antibody AO2268a
    Figure 1: Western blot analysis using GNL3 mAb against human GNL3 recombinant protein. (Expected MW is 51.9 kDa)
  • WB - GNL3 Antibody AO2268a
    Figure 2: Western blot analysis using GNL3 mAb against HEK293 (1) and GNL3 (AA: 1-226)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - GNL3 Antibody AO2268a
    Figure 3: Western blot analysis using GNL3 mouse mAb against NIH3T3 (1) and PC-3 (2) cell lysate.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9BVP2
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2C8D5
Isotype IgG1
Calculated MW 62kDa
Description The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene.
Immunogen Purified recombinant fragment of human GNL3 (AA: 1-226) expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 26354
Other Names Guanine nucleotide-binding protein-like 3, E2-induced gene 3 protein, Novel nucleolar protein 47, NNP47, Nucleolar GTP-binding protein 3, Nucleostemin, GNL3, E2IG3, NS
Dilution E~~1/10000
WB~~1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGNL3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GNL3
Synonyms E2IG3, NS
Function May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation (By similarity).
Cellular Location Nucleus. Nucleus, nucleolus. Note=Shuttles between the nucleus and nucleolus
Tissue Location Increased levels in lung tissue in cancer patients.
Research Areas
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1.Oncogene. 2011 Apr 7;30(14):1716-26. 2.J Cell Biol. 2009 Jun 1;185(5):827-39.

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$ 385.00
Cat# AO2268a
(40 western blots)
Availability: 7-10 days
Bulk Size
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