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FCER1A Antibody

Purified Mouse Monoclonal Antibody

     
  • E - FCER1A Antibody AO2284a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - FCER1A Antibody AO2284a
    Figure 1: Western blot analysis using FCER1A mAb against human FCER1A recombinant protein. (Expected MW is 32.5 kDa)
  • WB - FCER1A Antibody AO2284a
    Figure 2: Western blot analysis using FCER1A mouse mAb against mouse kidney tissue lysate.
  • FC - FCER1A Antibody AO2284a
    Figure 3: Flow cytometric analysis of HEK293 cells using FCER1A mouse mAb (green) and negative control (red).
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession P12319
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 1F2A9
Isotype IgG1
Calculated MW 29.6kDa
Description The immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine that are responsible for allergy symptoms are released. This receptor is comprised of an alpha subunit, a beta subunit, and two gamma subunits. The protein encoded by this gene represents the alpha subunit.
Immunogen Purified recombinant fragment of human FCER1A (AA:42-103) expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Other Names High affinity immunoglobulin epsilon receptor subunit alpha, Fc-epsilon RI-alpha, FcERI, IgE Fc receptor subunit alpha, FCER1A, FCE1A
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFCER1A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FCER1A
Synonyms FCE1A
Function Binds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines.
Cellular Location Cell membrane; Single-pass type I membrane protein EMBL; X06948; CAA30025.1; -; mRNA EMBL; J03605; AAA36204.1; -; mRNA EMBL; BC005912; AAH05912.1; -; mRNA CCDS; CCDS1184.1; - PIR; S00682; S00682 RefSeq; NP_001992.1; NM_002001.3 UniGene; Hs.897; - PDB; 1ALS; Model; -; A=26-197 PDB; 1ALT; Model; -; A=26-197 PDB; 1F2Q; X-ray; 2.40 A; A=26-201 PDB; 1F6A; X-ray; 3.50 A; A=26-201 PDB; 1J86; X-ray; 3.20 A; A/B=26-201 PDB; 1J87; X-ray; 3.20 A; A=26-197 PDB; 1J88; X-ray; 3.20 A; A/B/C/D/E=26-197 PDB; 1J89; X-ray; 4.10 A; A/B/C/D/E=26-197 PDB; 1RPQ; X-ray; 3.00 A; A/B/C/D=26-201 PDB; 2Y7Q; X-ray; 3.40 A; A=26-201 PDBsum; 1ALS; - PDBsum; 1ALT; - PDBsum; 1F2Q; - PDBsum; 1F6A; - PDBsum; 1J86; - PDBsum; 1J87; - PDBsum; 1J88; - PDBsum; 1J89; - PDBsum; 1RPQ; - PDBsum; 2Y7Q; - ProteinModelPortal; P12319; - SMR; P12319; - BioGrid; 108499; 29 DIP; DIP-6166N; - IntAct; P12319; 2 STRING; 9606.ENSP00000357097; - BindingDB; P12319; - ChEMBL; CHEMBL2248; - DrugBank; DB00895; Benzylpenicilloyl Polylysine DrugBank; DB00043; Omalizumab DrugBank; DB05797; TNX-901 GuidetoPHARMACOLOGY; 2933; - iPTMnet; P12319; - PhosphoSitePlus; P12319; - BioMuta; FCER1A; - DMDM; 119865; - PaxDb; P12319; - PeptideAtlas; P12319; - PRIDE; P12319; - ProteomicsDB; 52851; - DNASU; 2205; - Ensembl; ENST00000368115; ENSP00000357097; ENSG00000179639 GeneID; 2205; - KEGG; hsa:2205; - UCSC; uc001ftq.4; human CTD; 2205; - DisGeNET; 2205; - EuPathDB; HostDB:ENSG00000179639.10; - GeneCards; FCER1A; - HGNC; HGNC:3609; FCER1A HPA; CAB022102; - HPA; CAB055526; - HPA; HPA036051; - MIM; 147140; gene neXtProt; NX_P12319; - OpenTargets; ENSG00000179639; - PharmGKB; PA28056; - eggNOG; ENOG410IJME; Eukaryota eggNOG; ENOG410ZPGM; LUCA GeneTree; ENSGT00760000119130; - HOGENOM; HOG000251632; - HOVERGEN; HBG051602; - InParanoid; P12319; - KO; K08089; - OMA; YENHNIS; - OrthoDB; EOG091G0ETN; - PhylomeDB; P12319; - TreeFam; TF335097; - Reactome; R-HSA-2454202; Fc epsilon receptor (FCERI) signaling Reactome; R-HSA-2730905; Role of LAT2/NTAL/LAB on calcium mobilization Reactome; R-HSA-2871796; FCERI mediated MAPK activation Reactome; R-HSA-2871809; FCERI mediated Ca+2 mobilization Reactome; R-HSA-2871837; FCERI mediated NF-kB activation SIGNOR; P12319; - ChiTaRS; FCER1A; human EvolutionaryTrace; P12319; - GeneWiki; FCER1A; - GenomeRNAi; 2205; - PRO; PR:P12319; - Proteomes; UP000005640; Chromosome 1 Bgee; ENSG00000179639; - CleanEx; HS_FCER1A; - ExpressionAtlas; P12319; baseline and differential Genevisible; P12319; HS GO; GO:0009986; C:cell surface; IDA:UniProtKB GO; GO:0005887; C:integral component of plasma membrane; TAS:ProtInc GO; GO:0005886; C:plasma membrane; TAS:Reactome GO; GO:0019863; F:IgE binding; IEA:UniProtKB-KW GO; GO:0038095; P:Fc-epsilon receptor signaling pathway; TAS:Reactome Gene3D; 2.60.40.10; -; 2 InterPro; IPR007110; Ig-like_dom InterPro; IPR036179; Ig-like_dom_sf InterPro; IPR013783; Ig-like_fold InterPro; IPR003599; Ig_sub InterPro; IPR003598; Ig_sub2 Pfam; PF13895; Ig_2; 1 SMART; SM00409; IG; 2 SMART; SM00408; IGc2; 2 SUPFAM; SSF48726; SSF48726; 2 PROSITE; PS50835; IG_LIKE; 2 1: Evidence at protein level; 3D-structure; Cell membrane; Complete proteome; Direct protein sequencing; Disulfide bond; Glycoprotein; IgE-binding protein; Immunoglobulin domain; Membrane; Polymorphism; Receptor; Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix SIGNAL 1 25 CHAIN 26 257 High affinity immunoglobulin epsilon receptor subunit alpha /FTId=PRO_0000015161 TOPO_DOM 26 205 Extracellular. TRANSMEM 206 224 Helical. TOPO_DOM 225 257 Cytoplasmic. DOMAIN 30 110 Ig-like 1 DOMAIN 111 193 Ig-like 2 CARBOHYD 46 46 N-linked (GlcNAc...) asparagine CARBOHYD 67 67 N-linked (GlcNAc...) asparagine CARBOHYD 75 75 N-linked (GlcNAc...) asparagine CARBOHYD 99 99 N-linked (GlcNAc...) asparagine CARBOHYD 160 160 N-linked (GlcNAc...) asparagine CARBOHYD 165 165 N-linked (GlcNAc...) asparagine CARBOHYD 191 191 N-linked (GlcNAc...) asparagine DISULFID 51 93 {ECO:0000244|PDB:1F2Q, ECO:0000269|PubMed:11531339, ECO:0000269|PubMed:9875849} DISULFID 132 176 {ECO:0000244|PDB:1F2Q, ECO:0000269|PubMed:11531339, ECO:0000269|PubMed:9875849} VARIANT 84 84 K -> R (in dbSNP:rs2298804) /FTId=VAR_020091 VARIANT 101 101 S -> N (in dbSNP:rs2298805) /FTId=VAR_020092 STRAND 33 37 {ECO:0000244|PDB:1F2Q} STRAND 39 42 {ECO:0000244|PDB:1F2Q} STRAND 47 50 {ECO:0000244|PDB:1F2Q} STRAND 53 55 {ECO:0000244|PDB:1F6A} STRAND 63 66 {ECO:0000244|PDB:1F2Q} STRAND 69 72 {ECO:0000244|PDB:2Y7Q} STRAND 75 80 {ECO:0000244|PDB:1F2Q} HELIX 85 87 {ECO:0000244|PDB:1F2Q} STRAND 89 94 {ECO:0000244|PDB:1F2Q} STRAND 96 99 {ECO:0000244|PDB:1RPQ} STRAND 104 109 {ECO:0000244|PDB:1F2Q} STRAND 111 118 {ECO:0000244|PDB:1F2Q} STRAND 120 123 {ECO:0000244|PDB:1F2Q} STRAND 128 134 {ECO:0000244|PDB:1F2Q} HELIX 135 137 {ECO:0000244|PDB:1F2Q} STRAND 140 147 {ECO:0000244|PDB:1F2Q} STRAND 150 157 {ECO:0000244|PDB:1RPQ} STRAND 158 165 {ECO:0000244|PDB:1F2Q} HELIX 168 170 {ECO:0000244|PDB:1F2Q} STRAND 172 180 {ECO:0000244|PDB:1F2Q} STRAND 183 186 {ECO:0000244|PDB:1F2Q} STRAND 190 195 {ECO:0000244|PDB:1F2Q} SEQUENCE 257 AA; 29596 MW; F183BB2357DDAD58 CRC64; MAPAMESPTL LCVALLFFAP DGVLAVPQKP KVSLNPPWNR IFKGENVTLT CNGNNFFEVS STKWFHNGSL SEETNSSLNI VNAKFEDSGE YKCQHQQVNE SEPVYLEVFS DWLLLQASAE VVMEGQPLFL RCHGWRNWDV YKVIYYKDGE ALKYWYENHN ISITNATVED SGTYYCTGKV WQLDYESEPL NITVIKAPRE KYWLQFFIPL LVVILFAVDT GLFISTQQQV TFLLKIKRTR KGFRLLNPHP KPNPKNN
Research Areas
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References

1.Hum Immunol. 2012 Mar;73(3):301-5. 2.Mol Immunol. 2011 Apr;48(8):979-80.

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$ 385.00
Cat# AO2284a
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