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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   GUCY1A3 Antibody   

GUCY1A3 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - GUCY1A3 Antibody AO2286a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

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  • WB - GUCY1A3 Antibody AO2286a
    Figure 1: Western blot analysis using GUCY1A3 mAb against human GUCY1A3 recombinant protein. (Expected MW is 47.2 kDa)
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  • WB - GUCY1A3 Antibody AO2286a
    Figure 2: Western blot analysis using GUCY1A3 mAb against HEK293 (1) and GUCY1A3 (AA: 22-214)-hIgGFc transfected HEK293 (2) cell lysate.
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  • WB - GUCY1A3 Antibody AO2286a
    Figure 3: Western blot analysis using GUCY1A3 mouse mAb against HEK293 (1) and Jurkat (2) cell lysate.
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  • IF - GUCY1A3 Antibody AO2286a
    Figure 4: Immunofluorescence analysis of HepG2 cells using GUCY1A3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
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  • FC - GUCY1A3 Antibody AO2286a
    Figure 5: Flow cytometric analysis of HEK293 cells using GUCY1A3 mouse mAb (green) and negative control (purple).
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  • IHC - GUCY1A3 Antibody AO2286a
    Figure 6: Immunohistochemical analysis of paraffin-embedded renal cancer tissues using GUCY1A3 mouse mAb with DAB staining.
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  • IHC - GUCY1A3 Antibody AO2286a
    Figure 7: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using GUCY1A3 mouse mAb with DAB staining.
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, ICC, E
Primary Accession Q02108
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3G6B2
Isotype IgG1
Calculated MW 77.5kDa
Description Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene.
Immunogen Purified recombinant fragment of human GUCY1A3 (AA: 22-214) expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 2982
Other Names Guanylate cyclase soluble subunit alpha-3, GCS-alpha-3, 4.6.1.2, GCS-alpha-1, Soluble guanylate cyclase large subunit, GUCY1A3, GUC1A3, GUCSA3, GUCY1A1
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGUCY1A3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GUCY1A1 (HGNC:4685)
Cellular Location Cytoplasm.
Tissue Location Detected in brain cortex and lung (at protein level).
Research Areas
Citations (0)
citation

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References

1.Mol Endocrinol. 2012 Feb;26(2):292-307. 2.J Biol Inorg Chem. 2011 Dec;16(8):1227-39.

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$ 385.00
Cat# AO2286a
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