|Application ||WB, IHC, FC, E|
|Description||PIWIL4 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells|
|Immunogen||Purified recombinant fragment of human PIWIL4 (AA: 304-434) expressed in E. Coli.|
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Other Names||Piwi-like protein 4, PIWIL4, HIWI2, PIWI|
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PIWIL4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer- independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci.|
|Cellular Location||Nucleus. Cytoplasm. Note=Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. PIWIL2/MILI is required for nuclear localization (By similarity).|
|Tissue Location||Expressed in testis. According to PubMed:17544373, it is ubiquitously expressed|
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Provided below are standard protocols that you may find useful for product applications.
1.FEBS Lett. 2012 May 7;586(9):1356-62. 2.Hum Reprod. 2010 Dec;25(12):2955-61.
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