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PIWIL4 Antibody
Purified Mouse Monoclonal Antibody
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- CITATIONS
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- BACKGROUND
Application 
| WB, IHC, FC, E |
|---|---|
| Primary Accession | Q7Z3Z4 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 10G9B11 |
| Isotype | IgG1 |
| Calculated MW | 96.6kDa |
| Description | PIWIL4 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells |
| Immunogen | Purified recombinant fragment of human PIWIL4 (AA: 304-434) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Gene ID | 143689 |
|---|---|
| Other Names | Piwi-like protein 4, PIWIL4, HIWI2, PIWI |
| Dilution | E~~1/10000 WB~~1/500 - 1/2000 FC~~1/200 - 1/400 IHC~~1/200 - 1/1000 |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PIWIL4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PIWIL4 |
|---|---|
| Synonyms | HIWI2, PIWI |
| Function | Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (By similarity). Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements (By similarity). Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association (By similarity). The piRNA process acts upstream of known mediators of DNA methylation (By similarity). Does not show endonuclease activity (By similarity). Plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle, by acting as a 'slicer-incompetent' component that loads cleaved piRNAs from the 'slicer-competent' component PIWIL2 and target them on genomic transposon loci in the nucleus (By similarity). May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci (PubMed:17544373). In addition to its role in germline, PIWIL4 also plays a role in the regulation of somatic cells activities. Plays a role in pancreatic beta cell function and insulin secretion (By similarity). Involved in maintaining cell morphology and functional integrity of retinal epithelial through Akt/GSK3alpha/beta signaling pathway (PubMed:28025795). When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of cells proliferation in tumors (PubMed:22483988). |
| Cellular Location | Nucleus. Cytoplasm Note=Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. PIWIL2/MILI is required for nuclear localization (By similarity). {ECO:0000250|UniProtKB:Q8CGT6} |
| Tissue Location | Ubiquitously expressed (PubMed:25038252, PubMed:17544373, PubMed:28025795, PubMed:28711973, PubMed:22483988) Detected in retina, retinal pigment epithelia cells (RPE) (at protein level) (PubMed:28025795). |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
1.FEBS Lett. 2012 May 7;586(9):1356-62. 2.Hum Reprod. 2010 Dec;25(12):2955-61.
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$ 385.00
Cat# AO2293a
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