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ALDH2 Antibody

Purified Mouse Monoclonal Antibody

  • E - ALDH2 Antibody AO2295a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - ALDH2 Antibody AO2295a
    Figure 1: Western blot analysis using ALDH2 mAb against human ALDH2 recombinant protein. (Expected MW is 47.4 kDa)
  • WB - ALDH2 Antibody AO2295a
    Figure 2: Western blot analysis using ALDH2 mAb against HEK293 (1) and ALDH2 (AA: 317-517)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - ALDH2 Antibody AO2295a
    Figure 3: Western blot analysis using ALDH2 mouse mAb against HepG2 (1), A549 (2) cell lysate, and Rat live (3), Mouse liver (4), Mouse brain (5) tissue lysate.
  • IF - ALDH2 Antibody AO2295a
    Figure 4: Immunofluorescence analysis of HepG2 cells using ALDH2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
  • FC - ALDH2 Antibody AO2295a
    Figure 5: Flow cytometric analysis of HeLa cells using ALDH2 mouse mAb (green) and negative control (red).
  • IHC - ALDH2 Antibody AO2295a
    Figure 6: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ALDH2 mouse mAb with DAB staining.
  • IHC - ALDH2 Antibody AO2295a
    Figure 7: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ALDH2 mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P05091
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Clone Names 4G6A3
Isotype IgG1
Calculated MW 56.3kDa
Description This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Immunogen Purified recombinant fragment of human ALDH2 (AA: 317-517) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 217
Other Names Aldehyde dehydrogenase, mitochondrial,, ALDH class 2, ALDH-E2, ALDHI, ALDH2, ALDM
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALDH2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ALDH2
Synonyms ALDM
Cellular Location Mitochondrion matrix.
Research Areas
Citations (0)

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1.Eur Heart J. 2012 Jul;33(13):1606-14. 2.Clin Toxicol (Phila). 2012 Apr;50(4):242-9.

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$ 385.00
Cat# AO2295a
(40 western blots)
Availability: 7-10 days
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