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MRPL42 Antibody

Purified Mouse Monoclonal Antibody

  • E - MRPL42 Antibody AO2302a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - MRPL42 Antibody AO2302a
    Figure 1: Western blot analysis using MRPL42 mAb against human MRPL42 recombinant protein. (Expected MW is 37.9 kDa)
  • WB - MRPL42 Antibody AO2302a
    Figure 2: Western blot analysis using MRPL42 mAb against HEK293 (1) and MRPL42 (AA: 142-203)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - MRPL42 Antibody AO2302a
    Figure 3: Western blot analysis using MRPL42 mouse mAb against HL7702 (1), HepG2 (2), SMMC-7721 (3), HEK293 (4), HL60 (5), HeLa (6), and Raji (7) cell lysate.
  • IF - MRPL42 Antibody AO2302a
    Figure 4: Immunofluorescence analysis of HeLa cells using MRPL42 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
  • FC - MRPL42 Antibody AO2302a
    Figure 5: Flow cytometric analysis of HEK293 cells using MRPL42 mouse mAb (green) and negative control (purple).
  • IHC - MRPL42 Antibody AO2302a
    Figure 6: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using MRPL42 mouse mAb with DAB staining.
  • IHC - MRPL42 Antibody AO2302a
    Figure 7: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using MRPL42 mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y6G3
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3H6G11
Isotype IgG1
Calculated MW 16.7kDa
Description Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein identified as belonging to both the 28S and the 39S subunits. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 4q, 6p, 6q, 7p, and 15q.
Immunogen Purified recombinant fragment of human MRPL42 (AA: 142-203) expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 28977
Other Names 39S ribosomal protein L42, mitochondrial, L42mt, MRP-L42, 28S ribosomal protein S32, mitochondrial, MRP-S32, S32mt, 39S ribosomal protein L31, mitochondrial, L31mt, MRP-L31, MRPL42, MRPL31, MRPS32, RPML31
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMRPL42 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MRPL42
Synonyms MRPL31, MRPS32, RPML31
Cellular Location Mitochondrion.
Research Areas
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1.J Biol Chem. 2001 Nov 23;276(47):43958-69. 2.Genomics. 2003 May;81(5):468-80.

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$ 385.00
Cat# AO2302a
(40 western blots)
Availability: 7-10 days
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