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ALPI Antibody

Purified Mouse Monoclonal Antibody

  • E - ALPI Antibody AO2311a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - ALPI Antibody AO2311a
    Figure 1: Western blot analysis using CD33 mAb against human CD33 recombinant protein. (Expected MW is 49.2 kDa)
  • WB - ALPI Antibody AO2311a
    Figure 2: Western blot analysis using CD33 mAb against HEK293 (1) and CD33 (AA: 15-237)-hIgGFc transfected HEK293 (2) cell lysate.
  • FC - ALPI Antibody AO2311a
    Figure 5: Flow cytometric analysis of HepG2 cells using CD33 mouse mAb (green) and negative control (purple).
  • IHC - ALPI Antibody AO2311a
    Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CD33 mouse mAb with DAB staining.
  • IHC - ALPI Antibody AO2311a
    Figure 7: Immunohistochemical analysis of paraffin-embedded endometrium tissues using CD33 mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P09923
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3B10E7
Isotype IgG1
Calculated MW 39.8kDa
Description The protein encoded by this gene belongs to putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro) and CD33 plays potential key roles in the pathogenesis of Alzheimer's disease (AD)
Immunogen Purified recombinant fragment of human CD33 (AA: 15-237) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 248
Other Names Intestinal-type alkaline phosphatase, IAP, Intestinal alkaline phosphatase,, ALPI
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALPI Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Cellular Location Cell membrane; Lipid-anchor, GPI-anchor.
Research Areas
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1. MAbs. 2011 Jan-Feb;3(1):21-30. 2. Hum Genet. 2012 Jul;131(7):1245-9.

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$ 385.00
Cat# AO2311a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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