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PAX3 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - PAX3 Antibody AO2313a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

    detail
  • WB - PAX3 Antibody AO2313a
    Figure 1: Western blot analysis using ALPI mAb against human ALPI recombinant protein. (Expected MW is 31.9 kDa)
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  • WB - PAX3 Antibody AO2313a
    Figure 2: Western blot analysis using ALPI mAb against HEK293 (1) and ALPI (AA: 397-458)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  • WB - PAX3 Antibody AO2313a
    Figure 3: Western blot analysis using ALPI mouse mAb against HL60 (1) and HepG2 (2) cell lysate.
    detail
  • FC - PAX3 Antibody AO2313a
    Figure 5: Flow cytometric analysis of HepG2 cells using ALPI mouse mAb (green) and negative control (purple).
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  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P23760
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 7D8G7
Isotype IgG1
Calculated MW 56.8kDa
Description There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation.
Immunogen Purified recombinant fragment of human ALPI (AA: 397-458) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 5077
Other Names Paired box protein Pax-3, HuP2, PAX3, HUP2
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPAX3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PAX3
Synonyms HUP2
Function Transcription factor that may regulate cell proliferation, migration and apoptosis. Involved in neural development and myogenesis. Transcriptional activator of MITF, acting synergistically with SOX10 (PubMed:21965087).
Cellular Location Nucleus.
Research Areas
Citations (0)
citation

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References

1. Virchows Arch. 2012 Feb;460(2):157-61. 2. World J Gastroenterol. 2012 Jul 7;18(25):3254-9.

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$ 385.00
Cat# AO2313a
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