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TP53BP1 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - TP53BP1 Antibody AO2318a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - TP53BP1 Antibody AO2318a
    Figure 1: Western blot analysis using TP53BP1 mAb against human TP53BP1 recombinant protein. (Expected MW is 47.6 kDa)
  • WB - TP53BP1 Antibody AO2318a
    Figure 2: Western blot analysis using TP53BP1 mAb against HEK293 (1) and TP53BP1 (AA: 574-773)-hIgGFc transfected HEK293 (2) cell lysate.
  • FC - TP53BP1 Antibody AO2318a
    Figure 3: Flow cytometric analysis of HepG2 cells using TP53BP1 mouse mAb (green) and negative control (purple).
  • IHC - TP53BP1 Antibody AO2318a
    Figure 4: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using TP53BP1 mouse mAb with DAB staining.
  • IHC - TP53BP1 Antibody AO2318a
    Figure 5: Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using TP53BP1 mouse mAb with DAB staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, E
Primary Accession Q12888
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6B3E10
Isotype IgG1
Calculated MW 213.6kDa
Description T protein p53 binding protein 1 may have a role in checkpoint signaling during mitosis,enhance TP53-mediated transcriptional activation and play a role in the response to DNA damage.
Immunogen Purified recombinant fragment of human TP53BP1 (AA: 574-773) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 7158
Other Names Tumor suppressor p53-binding protein 1, 53BP1, p53-binding protein 1, p53BP1, TP53BP1
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTP53BP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TP53BP1 (HGNC:11999)
Function Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis (PubMed:12364621, PubMed:22553214, PubMed:23333306, PubMed:17190600, PubMed:21144835, PubMed:28241136). Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1 (PubMed:22553214, PubMed:23727112, PubMed:23333306). In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites (PubMed:28241136). Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:23760478, PubMed:28241136, PubMed:17190600). Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed:23345425). Participates to the repair and the orientation of the broken DNA ends during CSR (By similarity). In contrast, it is not required for classic NHEJ and V(D)J recombination (By similarity). Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1 (PubMed:23727112).
Cellular Location Nucleus Chromosome Chromosome, centromere, kinetochore {ECO:0000250|UniProtKB:P70399}. Note=Localizes to the nucleus in absence of DNA damage (PubMed:28241136). Following DNA damage, recruited to sites of DNA damage, such as double stand breaks (DSBs): recognizes and binds histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:23333306, PubMed:23760478, PubMed:24703952, PubMed:28241136, PubMed:17190600). Associated with kinetochores during mitosis (By similarity). {ECO:0000250|UniProtKB:P70399, ECO:0000269|PubMed:17190600, ECO:0000269|PubMed:23333306, ECO:0000269|PubMed:23760478, ECO:0000269|PubMed:28241136}
Research Areas
Citations (0)

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References

1. Cancer Res. 2012 Oct 1;72(19):4974-83. 2. Int J Biochem Cell Biol. 2012 Sep;44(9):1398-409.

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$ 385.00
Cat# AO2318a
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