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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   EGFR mutant Antibody   

EGFR mutant Antibody

Purified Mouse Monoclonal Antibody

  • E - EGFR mutant Antibody AO2321a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - EGFR mutant Antibody AO2321a
    Figure 1: Western blot analysis using EGFR mutant mAb against human EGFR mutant recombinant protein. (Expected MW is 36.9 kDa)
  • WB - EGFR mutant Antibody AO2321a
    Figure 2: Western blot analysis using EGFR mutant mAb against HEK293 (1) and EGFR mutant (AA: 693-893)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - EGFR mutant Antibody AO2321a
    Figure 3: Western blot analysis using EGFR mutant mouse mAb against SPC-A-1 (1), A549 (2), HepG2 (3) and MCF-7 (4) cell lysate.
  • FC - EGFR mutant Antibody AO2321a
    Figure 4: Flow cytometric analysis of HepG2 cells using EGFR mutant mouse mAb (green) and negative control (purple).
  • IHC - EGFR mutant Antibody AO2321a
    Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using EGFR mutant mouse mAb with DAB staining.
  • IHC - EGFR mutant Antibody AO2321a
    Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using EGFR mutant mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P00533
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5G9B5
Isotype IgG1
Calculated MW 175kDa
Description The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.
Immunogen Purified recombinant fragment of human EGFR mutant (AA: 693-893) expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 1956
Other Names Epidermal growth factor receptor,, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEGFR mutant Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms ERBB, ERBB1, HER1
Function Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Cellular Location Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Nucleus. Note=In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF)
Tissue Location Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
Research Areas
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1. J Immunol. 2012 Dec 1;189(11):5230-9. 2. J Biol Chem. 2012 Oct 12;287(42):35201-11.

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$ 385.00
Cat# AO2321a
(40 western blots)
Availability: 7-10 days
Bulk Size
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