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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Mouse Monoclonal Antibody to DNMT3L   

Mouse Monoclonal Antibody to DNMT3L

Purified Mouse Monoclonal Antibody

     
  • E - Mouse Monoclonal Antibody to DNMT3L AO2324a
    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • WB - Mouse Monoclonal Antibody to DNMT3L AO2324a
    Western blot analysis using DNMT3L mAb against human DNMT3L (AA: 147-386) recombinant protein. (Expected MW is 53.9 kDa)
  • WB - Mouse Monoclonal Antibody to DNMT3L AO2324a
    Western blot analysis using DNMT3L mAb against HEK293 (1) and DNMT3L (AA: 147-386)-hIgGFc transfected HEK293 (2) cell lysate.
  • FC - Mouse Monoclonal Antibody to DNMT3L AO2324a
    Flow cytometric analysis of Hela cells using DNMT3L mouse mAb (green) and negative control (red).
  • FC - Mouse Monoclonal Antibody to DNMT3L AO2324a
    Flow cytometric analysis of HepG2 cells using DNMT3L mouse mAb (green) and negative control (red).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q9UJW3
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2D10H11
Isotype Mouse IgG1
Calculated MW 43.6kDa
Description CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.;
Immunogen Purified recombinant fragment of human DNMT3L (AA: 147-386) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application Note ELISA: 1/10000
WB: 1/500 - 1/2000
FCM: 1/200 - 1/400
Additional Information
Gene ID 29947
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Monoclonal Antibody to DNMT3L is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DNMT3L
Function Catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation depending on the context (By similarity). Essential for the function of DNMT3A and DNMT3B: activates DNMT3A and DNMT3B by binding to their catalytic domain (PubMed:17687327). Acts by accelerating the binding of DNA and S-adenosyl-L-methionine (AdoMet) to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases (PubMed:17687327). Recognizes unmethylated histone H3 lysine 4 (H3K4me0) and induces de novo DNA methylation by recruitment or activation of DNMT3 (PubMed:17687327). Plays a key role in embryonic stem cells and germ cells (By similarity). In germ cells, required for the methylation of imprinted loci together with DNMT3A (By similarity). In male germ cells, specifically required to methylate retrotransposons, preventing their mobilization (By similarity). Plays a key role in embryonic stem cells (ESCs) by acting both as an positive and negative regulator of DNA methylation (By similarity). While it promotes DNA methylation of housekeeping genes together with DNMT3A and DNMT3B, it also acts as an inhibitor of DNA methylation at the promoter of bivalent genes (By similarity). Interacts with the EZH2 component of the PRC2/EED-EZH2 complex, preventing interaction of DNMT3A and DNMT3B with the PRC2/EED-EZH2 complex, leading to maintain low methylation levels at the promoters of bivalent genes (By similarity). Promotes differentiation of ESCs into primordial germ cells by inhibiting DNA methylation at the promoter of RHOX5, thereby activating its expression (By similarity).
Cellular Location Nucleus.
Tissue Location Expressed at low levels in several tissues including testis, ovary, and thymus
Research Areas
Citations (0)

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References

1.Clin Cancer Res. 2010 May 15;16(10):2751-9. ; 2.Epigenetics. 2009 Jul 1;4(5):322-9. ;

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$ 385.00
Cat# AO2324a
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