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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Mouse Monoclonal Antibody to YWHAB   

Mouse Monoclonal Antibody to YWHAB

Purified Mouse Monoclonal Antibody

     
  • E - Mouse Monoclonal Antibody to YWHAB AO2325a
    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • WB - Mouse Monoclonal Antibody to YWHAB AO2325a
    Western blot analysis using YWHAB mAb against human YWHAB (AA: 1-246) recombinant protein. (Expected MW is 54 kDa)
  • WB - Mouse Monoclonal Antibody to YWHAB AO2325a
    Western blot analysis using YWHAB mAb against HEK293 (1) and YWHAB (AA: 1-246)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - Mouse Monoclonal Antibody to YWHAB AO2325a
    Western blot analysis using YWHAB mouse mAb against Hela (1), NIH/3T3 (2), C6 (3), A431 (4), K562 (5), PC-12 (6), and U937 (7) cell lysate.
  • FC - Mouse Monoclonal Antibody to YWHAB AO2325a
    Flow cytometric analysis of Hela cells using YWHAB mouse mAb (green) and negative control (red).
  • ICC - Mouse Monoclonal Antibody to YWHAB AO2325a
    Immunofluorescence analysis of GC-7901 cells using YWHAB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher
  • ICC - Mouse Monoclonal Antibody to YWHAB AO2325a
    Immunofluorescence analysis of Hela cells using YWHAB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, ICC, E
Primary Accession P31946
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Clone Names 5B5G10
Isotype Mouse IgG2b
Calculated MW 28kDa
Description This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene.;
Immunogen Purified recombinant fragment of human YWHAB (AA: 1-246) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application Note ELISA: 1/10000
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/200 - 1/1000
FCM: 1/200 - 1/400
Additional Information
Gene ID 7529
Other Names HS1; GW128; YWHAA; KCIP-1; HEL-S-1
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Monoclonal Antibody to YWHAB is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name YWHAB
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.
Cellular Location Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Research Areas
Citations (0)

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References

1.BMC Res Notes. 2014 Feb 20;7:97. ; 2.Mol Biol Rep. 2012 Dec;39(12):10647-53.;

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$ 385.00
Cat# AO2325a
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