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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Mouse Monoclonal Antibody to LMNB2   

Mouse Monoclonal Antibody to LMNB2

Purified Mouse Monoclonal Antibody

     
  • E - Mouse Monoclonal Antibody to LMNB2 AO2338a
    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • WB - Mouse Monoclonal Antibody to LMNB2 AO2338a
    Western blot analysis using LMNB2 mAb against human LMNB2 (AA: 401-600) recombinant protein. (Expected MW is 47.6 kDa)
  • WB - Mouse Monoclonal Antibody to LMNB2 AO2338a
    Western blot analysis using LMNB2 mAb against HEK293 (1) and LMNB2 (AA: 401-600)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - Mouse Monoclonal Antibody to LMNB2 AO2338a
    Western blot analysis using LMNB2 mouse mAb against PC-3 (1), LNcap (2), Jurkat (3), and HCT116 (4) cell lysate.
  • FC - Mouse Monoclonal Antibody to LMNB2 AO2338a
    Flow cytometric analysis of Hela cells using LMNB2 mouse mAb (green) and negative control (red).
  • ICC - Mouse Monoclonal Antibody to LMNB2 AO2338a
    Immunofluorescence analysis of GC-7901 cells using LMNB2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher
  • ICC - Mouse Monoclonal Antibody to LMNB2 AO2338a
    Immunofluorescence analysis of Hela cells using LMNB2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, ICC, E
Primary Accession Q03252
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2E2F4
Isotype Mouse IgG2b
Calculated MW 67.7kDa
Description This gene encodes a B type nuclear lamin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Mutations in this gene are associated with acquired partial lipodystrophy.;
Immunogen Purified recombinant fragment of human LMNB2 (AA: 401-600) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application Note ELISA: 1/10000
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/200 - 1/1000
FCM: 1/200 - 1/400
Additional Information
Gene ID 84823
Other Names LMN2; LAMB2
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Monoclonal Antibody to LMNB2 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LMNB2
Synonyms LMN2
Function Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Cellular Location Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side
Research Areas
Citations (0)

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References

1.J Pediatr Endocrinol Metab. 2012;25(3-4):375-7. ; 2.FEBS Lett. 2006 Nov 13;580(26):6211-6. ;

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$ 385.00
Cat# AO2338a
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