|Application ||WB, IHC, E|
|Description||This gene encodes a 5'-3' exonuclease that promotes transcription termination at cotranscriptional cleavage sites. Alternative splicing results in multiple transcript variants encoding different isoforms.;|
|Immunogen||Purified recombinant fragment of human XRN2 (AA: 398-547) expressed in E. Coli.|
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Application Note||ELISA: 1/10000|
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Mouse Monoclonal Antibody to XRN2 is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Possesses 5'->3' exoribonuclease activity (By similarity). May promote the termination of transcription by RNA polymerase II. During transcription termination, cleavage at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription. Binds to RNA polymerase II (RNAp II) transcription termination R-loops formed by G-rich pause sites (PubMed:21700224).|
|Cellular Location||Nucleus, nucleolus|
|Tissue Location||Expressed in the spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Isoform 2 is expressed predominantly in peripheral blood leukocytes.|
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1.EMBO J. 2012 May 30;31(11):2566-78. ; 2.DNA Seq. 2005 Apr;16(2):143-6.;
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