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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Mouse Monoclonal Antibody to DHX58   

Mouse Monoclonal Antibody to DHX58

Purified Mouse Monoclonal Antibody

     
  • E - Mouse Monoclonal Antibody to DHX58 AO2347a
    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • WB - Mouse Monoclonal Antibody to DHX58 AO2347a
    Western blot analysis using DHX58 mAb against human DHX58 (AA: 479-678) recombinant protein. (Expected MW is 48.7 kDa)
  • WB - Mouse Monoclonal Antibody to DHX58 AO2347a
    Western blot analysis using DHX58 mAb against HEK293 (1) and DHX58 (AA: 479-678)-hIgGFc transfected HEK293 (2) cell lysate.
  • FC - Mouse Monoclonal Antibody to DHX58 AO2347a
    Flow cytometric analysis of Hela cells using DHX58 mouse mAb (green) and negative control (red).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q96C10
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3E11F1
Isotype Mouse IgG1
Calculated MW 76.6kDa
Description DHX58 (DEXH (Asp-Glu-X-His) box polypeptide 58) is a protein-coding gene. GO annotations related to this gene include single-stranded RNA binding and helicase activity. An important paralog of this gene is IFIH1.;
Immunogen Purified recombinant fragment of human DHX58 (AA: 479-678) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application Note ELISA: 1/10000
WB: 1/500 - 1/2000
FCM: 1/200 - 1/400
Additional Information
Gene ID 79132
Other Names LGP2; RLR-3; D11LGP2; D11lgp2e
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Monoclonal Antibody to DHX58 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DHX58
Synonyms D11LGP2E, LGP2
Function Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1- dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA, binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate.
Cellular Location Cytoplasm.
Research Areas
Citations (0)

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References

1.J Biol Chem. 2009 May 15;284(20):13881-91. ; 2.J Biol Chem. 2008 Jun 6;283(23):15825-33.;

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$ 385.00
Cat# AO2347a
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