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Mouse Monoclonal Antibody to PLD2

Purified Mouse Monoclonal Antibody

     
  • E - Mouse Monoclonal Antibody to PLD2 AO2360a
    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
    detail
  • WB - Mouse Monoclonal Antibody to PLD2 AO2360a
    Western blot analysis using PLD2 mAb against human PLD2 (AA: 834-933) recombinant protein. (Expected MW is 37.4 kDa)
    detail
  • WB - Mouse Monoclonal Antibody to PLD2 AO2360a
    Western blot analysis using PLD2 mAb against HEK293 (1) and PLD2 (AA: 834-933)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  • FC - Mouse Monoclonal Antibody to PLD2 AO2360a
    Flow cytometric analysis of Hela cells using PLD2 mouse mAb (green) and negative control (red).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession O14939
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 8G1G2
Isotype Mouse IgG1
Calculated MW 106kDa
Description The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.;
Immunogen Purified recombinant fragment of human PLD2 (AA: 834-933) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application Note ELISA: 1/10000
WB: 1/500 - 1/2000
FCM: 1/200 - 1/400
Additional Information
Gene ID 5338
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Monoclonal Antibody to PLD2 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PLD2 (HGNC:9068)
Function Function as phospholipase selective for phosphatidylcholine (PubMed:9582313). May have a role in signal-induced cytoskeletal regulation and/or endocytosis (By similarity).
Cellular Location Cell membrane {ECO:0000250|UniProtKB:P97813}; Lipid-anchor {ECO:0000250|UniProtKB:P97813}
Tissue Location Ubiquitous..
Research Areas
Citations (0)
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References

1.Exp Mol Med. 2014 Dec 5;46:e124. ; 2.FEBS Lett. 2014 Aug 25;588(17):3251-8.;

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$ 385.00
Cat# AO2360a
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