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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Mouse Monoclonal Antibody to PGRMC1   

Mouse Monoclonal Antibody to PGRMC1

Purified Mouse Monoclonal Antibody

     
  • E - Mouse Monoclonal Antibody to PGRMC1 AO2363a
    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • WB - Mouse Monoclonal Antibody to PGRMC1 AO2363a
    Western blot analysis using PGRMC1 mAb against human PGRMC1 (AA: 1-195) recombinant protein. (Expected MW is 47.6 kDa)
  • WB - Mouse Monoclonal Antibody to PGRMC1 AO2363a
    Western blot analysis using PGRMC1 mAb against HEK293 (1) and PGRMC1 (AA: 1-195)-hIgGFc transfected HEK293 (2) cell lysate.
  • FC - Mouse Monoclonal Antibody to PGRMC1 AO2363a
    Flow cytometric analysis of A549 cells using PGRMC1 mouse mAb (green) and negative control (red).
  • ICC - Mouse Monoclonal Antibody to PGRMC1 AO2363a
    Immunofluorescence analysis of SK-OV-3 cells using PGRMC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher
  • IHC - Mouse Monoclonal Antibody to PGRMC1 AO2363a
    Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using PGRMC1 mouse mAb with DAB staining.
  • IHC - Mouse Monoclonal Antibody to PGRMC1 AO2363a
    Immunohistochemical analysis of paraffin-embedded breast cancer tissues using PGRMC1 mouse mAb with DAB staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, ICC, E
Primary Accession O00264
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7G11G8
Isotype Mouse IgG1
Calculated MW 21.7kDa
Description This gene encodes a putative membrane-associated progesterone steroid receptor. The protein is expressed predominantly in the liver and kidney.;
Immunogen Purified recombinant fragment of human PGRMC1 (AA: 1-195) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application Note ELISA: 1/10000
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/200 - 1/1000
FCM: 1/200 - 1/400
Additional Information
Gene ID 10857
Other Names MPR; HPR6.6
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Monoclonal Antibody to PGRMC1 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PGRMC1 (HGNC:16090)
Synonyms HPR6.6, PGRMC
Function Component of a progesterone-binding protein complex (PubMed:28396637). Binds progesterone (PubMed:25675345). Has many reported cellular functions (heme homeostasis, interaction with CYPs).
Cellular Location Microsome membrane {ECO:0000250|UniProtKB:Q95250}; Single-pass membrane protein. Smooth endoplasmic reticulum membrane; Single-pass membrane protein
Tissue Location Widely expressed, with highest expression in liver and kidney
Research Areas
Citations (0)

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References

1.Cancer Lett. 2015 Jan 28;356(2 Pt B):434-42. ; 2.Nan Fang Yi Ke Da Xue Xue Bao. 2012 May;32(5):635-8. ;

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$ 385.00
Cat# AO2363a
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