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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Mouse Monoclonal Antibody to SMAD1   

Mouse Monoclonal Antibody to SMAD1

Purified Mouse Monoclonal Antibody

     
  • E - Mouse Monoclonal Antibody to SMAD1 AO2364a
    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • WB - Mouse Monoclonal Antibody to SMAD1 AO2364a
    Western blot analysis using SMAD1 mAb against human SMAD1 (AA: 1-110) recombinant protein. (Expected MW is 38.5 kDa)
  • WB - Mouse Monoclonal Antibody to SMAD1 AO2364a
    Western blot analysis using SMAD1 mAb against HEK293 (1) and SMAD1 (AA: 1-110)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - Mouse Monoclonal Antibody to SMAD1 AO2364a
    Western blot analysis using SMAD1 mouse mAb against NIH/3T3 (1), COS7 (2), HUVEC (3), and C2C12 (4) cell lysate.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q15797
Reactivity Human, Mouse, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 4E12H10
Isotype Mouse IgG1
Calculated MW 52kDa
Description The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.;
Immunogen Purified recombinant fragment of human SMAD1 (AA: 1-110) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application Note ELISA: 1/10000
WB: 1/500 - 1/2000
Additional Information
Gene ID 4086
Other Names BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Monoclonal Antibody to SMAD1 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SMAD1
Synonyms BSP1, MADH1, MADR1
Function Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.
Cellular Location Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4 (PubMed:15647271). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15647271). Exported from the nucleus to the cytoplasm when dephosphorylated (By similarity) {ECO:0000250|UniProtKB:P70340, ECO:0000269|PubMed:15647271}
Tissue Location Ubiquitous. Highest expression seen in the heart and skeletal muscle
Research Areas
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References

1.Histol Histopathol. 2011 Apr;26(4):531-41. ; 2.Blood. 2011 Jun 16;117(24):6489-97.;

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$ 385.00
Cat# AO2364a
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