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> home > Products > Primary Antibodies > Antibody Collections > Anti-Wnt-Pathway (plus Lgr5) Antibodies > Mouse Monoclonal Antibody to SMAD1
Mouse Monoclonal Antibody to SMAD1
Purified Mouse Monoclonal Antibody
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Application 
| WB, E |
|---|---|
| Primary Accession | Q15797 |
| Reactivity | Human, Mouse, Monkey |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 4E12H10 |
| Isotype | Mouse IgG1 |
| Calculated MW | 52kDa |
| Description | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.; |
| Immunogen | Purified recombinant fragment of human SMAD1 (AA: 1-110) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Application Note | ELISA: 1/10000 WB: 1/500 - 1/2000 |
| Gene ID | 4086 |
|---|---|
| Other Names | BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1 |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Mouse Monoclonal Antibody to SMAD1 is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SMAD1 |
|---|---|
| Synonyms | BSP1, MADH1, MADR1 |
| Function | Transcriptional modulator that plays a role in various cellular processes, including embryonic development, cell differentiation, and tissue homeostasis (PubMed:9335504). Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form an heteromeric complex which translocates into the nucleus acting as transcription factor (PubMed:33667543). In turn, the hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate the outcome of the signaling network (PubMed:33667543). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. Positively regulates BMP4-induced expression of odontogenic development regulator MSX1 following IPO7-mediated nuclear import (By similarity). |
| Cellular Location | Cytoplasm. Nucleus Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4 (PubMed:15647271). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15647271). Exported from the nucleus to the cytoplasm when dephosphorylated (By similarity) {ECO:0000250|UniProtKB:P70340, ECO:0000269|PubMed:15647271} |
| Tissue Location | Ubiquitous. Highest expression seen in the heart and skeletal muscle |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
1.Histol Histopathol. 2011 Apr;26(4):531-41. ; 2.Blood. 2011 Jun 16;117(24):6489-97.;
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$ 385.00
Cat# AO2364a
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