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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Mouse Monoclonal Antibody to VIMP   

Mouse Monoclonal Antibody to VIMP

Purified Mouse Monoclonal Antibody

     
  • E - Mouse Monoclonal Antibody to VIMP AO2395a
    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
    detail
  • WB - Mouse Monoclonal Antibody to VIMP AO2395a
    Western blot analysis using VIMP mAb against human VIMP (AA: 1-187) recombinant protein. (Expected MW is 46.9 kDa)
    detail
  • WB - Mouse Monoclonal Antibody to VIMP AO2395a
    Western blot analysis using VIMP mAb against HEK293 (1) and VIMP (AA: 1-187)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  • FC - Mouse Monoclonal Antibody to VIMP AO2395a
    Flow cytometric analysis of Hela cells using VIMP mouse mAb (green) and negative control (red).
    detail
  • ICC - Mouse Monoclonal Antibody to VIMP AO2395a
    Immunofluorescence analysis of Hela cells using VIMP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC, E
Primary Accession Q9BQE4
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5G4A10
Isotype Mouse IgG1
Calculated MW 21.2kDa
Description This gene encodes a member of the selenoprotein family, characterized by a selenocysteine (Sec) residue at the active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Studies suggest that this protein may regulate cytokine production, and thus play a key role in the control of the inflammatory response. Alternative splicing results in multiple transcript variants encoding different isoforms.;
Immunogen Purified recombinant fragment of human VIMP (AA: 1-187) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application Note ELISA: 1/10000
WB: 1/500 - 1/2000
ICC: 1/50 - 1/250
FCM: 1/200 - 1/400
Additional Information
Gene ID 55829
Other Names SELS; ADO15; SBBI8; SEPS1; AD-015
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Monoclonal Antibody to VIMP is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SELENOS {ECO:0000303|PubMed:27645994, ECO:0000312|HGNC:HGNC:30396}
Function Involved in the degradation process of misfolded endoplasmic reticulum (ER) luminal proteins. Participates in the transfer of misfolded proteins from the ER to the cytosol, where they are destroyed by the proteasome in a ubiquitin-dependent manner. Probably acts by serving as a linker between DERL1, which mediates the retrotranslocation of misfolded proteins into the cytosol, and the ATPase complex VCP, which mediates the translocation and ubiquitination.
Cellular Location Endoplasmic reticulum membrane; Single-pass membrane protein. Cytoplasm
Research Areas
Citations (0)
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References

1.Free Radic Biol Med. 2014 Feb;67:265-77. ; 2.PLoS One. 2013 Jun 11;8(6):e65657.;

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$ 385.00
Cat# AO2395a
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