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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Mouse Monoclonal Antibody to TRIM25   

Mouse Monoclonal Antibody to TRIM25

Purified Mouse Monoclonal Antibody

     
  • E - Mouse Monoclonal Antibody to TRIM25 AO2410a
    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • WB - Mouse Monoclonal Antibody to TRIM25 AO2410a
    Western blot analysis using TRIM25 mAb against human TRIM25 (AA: 211-360) recombinant protein. (Expected MW is 43.5 kDa)
  • WB - Mouse Monoclonal Antibody to TRIM25 AO2410a
    Western blot analysis using TRIM25 mAb against HEK293 (1) and TRIM25 (AA: 211-360)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - Mouse Monoclonal Antibody to TRIM25 AO2410a
    Western blot analysis using TRIM25 mouse mAb against MCF-7 (1), MCF-7 (2), K562 (3), A549 (4), and MOLT4 (5) cell lysate.
  • ICC - Mouse Monoclonal Antibody to TRIM25 AO2410a
    Immunofluorescence analysis of Hela cells using TRIM25 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher
  • FC - Mouse Monoclonal Antibody to TRIM25 AO2410a
    Flow cytometric analysis of Hela cells using TRIM25 mouse mAb (green) and negative control (red).
  • IHC - Mouse Monoclonal Antibody to TRIM25 AO2410a
    Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using TRIM25 mouse mAb with DAB staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, ICC, E
Primary Accession Q14258
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5B5B12
Isotype Mouse IgG2b
Calculated MW 71kDa
Description The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.;
Immunogen Purified recombinant fragment of human TRIM25 (AA: 211-360) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application Note ELISA: 1/10000
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/50 - 1/250
FCM: 1/200 - 1/400
Additional Information
Gene ID 7706
Other Names EFP; Z147; RNF147; ZNF147
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Monoclonal Antibody to TRIM25 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TRIM25
Synonyms EFP, RNF147, ZNF147
Function Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3 (PubMed:22452784).
Cellular Location Cytoplasm. Note=Colocalized with DDX58 at cytoplasmic perinuclear bodies
Tissue Location Expressed in breast tumors (at protein level). Ubiquitous.
Research Areas
Citations (0)

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References

1.Science. 2015 Oct 9;350(6257):217-21. ; 2.Oncogene. 2015 Nov 12;34(46):5729-38.;

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$ 385.00
Cat# AO2410a
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