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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Mouse Monoclonal Antibody to ZFP91   

Mouse Monoclonal Antibody to ZFP91

Purified Mouse Monoclonal Antibody

     
  • E - Mouse Monoclonal Antibody to ZFP91 AO2417a
    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • WB - Mouse Monoclonal Antibody to ZFP91 AO2417a
    Western blot analysis using ZFP91 mAb against human ZFP91 (AA: 162-304) recombinant protein. (Expected MW is 43 kDa)
  • WB - Mouse Monoclonal Antibody to ZFP91 AO2417a
    Western blot analysis using ZFP91 mAb against HEK293 (1) and ZFP91 (AA: 162-304)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - Mouse Monoclonal Antibody to ZFP91 AO2417a
    Western blot analysis using ZFP91 mouse mAb against Jurkat (1), A431 (2), HepG2 (3), HEK293 (4), A549 (5), and PC-3 (6) cell lysate.
  • ICC - Mouse Monoclonal Antibody to ZFP91 AO2417a
    Immunofluorescence analysis of Hela cells using ZFP91 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • ICC - Mouse Monoclonal Antibody to ZFP91 AO2417a
    Immunofluorescence analysis of Hela cells using ZFP91 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher
  • FC - Mouse Monoclonal Antibody to ZFP91 AO2417a
    Flow cytometric analysis of Hela cells using ZFP91 mouse mAb (green) and negative control (red).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, ICC, E
Primary Accession Q96JP5
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 8C3D5
Isotype Mouse IgG1
Calculated MW 63.4kDa
Description The protein encoded by this gene is a member of the zinc finger family of proteins. The gene product contains C2H2-type domains, which are the classical zinc finger domains found in numerous nucleic acid-binding proteins. This protein functions as a regulator of the non-canonical NF-kappaB pathway in lymphotoxin-beta receptor signaling. Alternative splicing results in multiple transcript variants. A read-through transcript variant composed of ZFP91 and the downstream CNTF gene sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse. A ZFP91-related pseudogene has also been identified on chromosome 2.;
Immunogen Purified recombinant fragment of human ZFP91 (AA: 162-304) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application Note ELISA: 1/10000
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/100- 1/500
FCM: 1/200 - 1/400
Additional Information
Gene ID 80829
Other Names PZF; DMS-8; DSM-8; FKSG11; ZFP-91; ZNF757
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Monoclonal Antibody to ZFP91 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ZFP91
Synonyms ZNF757
Function Atypical E3 ubiquitin-protein ligase that mediates 'Lys- 63'-linked ubiquitination of MAP3K14/NIK, leading to stabilize and activate MAP3K14/NIK. It thereby acts as an activator of the non- canonical NF-kappa-B2/NFKB2 pathway. May also play an important role in cell proliferation and/or anti-apoptosis.
Cellular Location Nucleus.
Tissue Location Expressed ubiquitously, particularly at high level in testis. Isoform 2 is testis specific
Research Areas
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References

1.Pathol Oncol Res. 2014 Apr;20(2):453-9. ; 2.Biochem Biophys Res Commun. 2010 Oct 1;400(4):581-6.;

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$ 385.00
Cat# AO2417a
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