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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Mouse Monoclonal Antibody to KDM3A   

Mouse Monoclonal Antibody to KDM3A

Purified Mouse Monoclonal Antibody

     
  • E - Mouse Monoclonal Antibody to KDM3A AO2425a
    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • WB - Mouse Monoclonal Antibody to KDM3A AO2425a
    Western blot analysis using KDM3A mAb against human KDM3A (AA: 311-445) recombinant protein. (Expected MW is 40.3 kDa)
  • WB - Mouse Monoclonal Antibody to KDM3A AO2425a
    Western blot analysis using KDM3A mAb against HEK293 (1) and KDM3A (AA: 311-445)-hIgGFc transfected HEK293 (2) cell lysate.
  • ICC - Mouse Monoclonal Antibody to KDM3A AO2425a
    Immunofluorescence analysis of Hela cells using KDM3A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher
  • FC - Mouse Monoclonal Antibody to KDM3A AO2425a
    Flow cytometric analysis of Hela cells using KDM3A mouse mAb (green) and negative control (red).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC, E
Primary Accession Q9Y4C1
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3G10C2
Isotype Mouse IgG1
Calculated MW 147kDa
Description This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants.;
Immunogen Purified recombinant fragment of human KDM3A (AA: 311-445) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application Note ELISA: 1/10000
WB: 1/500 - 1/2000
ICC: 1/50 - 1/250
FCM: 1/200 - 1/400
Additional Information
Gene ID 55818
Other Names TSGA; JMJD1; JHDM2A; JHMD2A; JMJD1A
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Monoclonal Antibody to KDM3A is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KDM3A
Synonyms JHDM2A, JMJD1, JMJD1A, KIAA0742, TSGA
Function Histone demethylase that specifically demethylates 'Lys- 9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TNP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
Cellular Location Cytoplasm. Nucleus. Note=Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa (By similarity).
Research Areas
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References

1.PLoS Biol. 2014 Dec 23;12(12):e1002026. ; 2.Oncotarget. 2014 Apr 15;5(7):1793-804.;

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$ 385.00
Cat# AO2425a
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