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Mouse Monoclonal Antibody to MIB1

Purified Mouse Monoclonal Antibody

     
  • E - Mouse Monoclonal Antibody to MIB1 AO2430a
    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • WB - Mouse Monoclonal Antibody to MIB1 AO2430a
    Western blot analysis using MIB1 mAb against human MIB1 (AA: 6-221) recombinant protein. (Expected MW is 50.1 kDa)
  • WB - Mouse Monoclonal Antibody to MIB1 AO2430a
    Western blot analysis using MIB1 mAb against HEK293 (1) and MIB1 (AA: 6-221)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - Mouse Monoclonal Antibody to MIB1 AO2430a
    Western blot analysis using MIB1 mouse mAb against Hela (1) and COS7 (2) cell lysate.
  • FC - Mouse Monoclonal Antibody to MIB1 AO2430a
    Flow cytometric analysis of Hela cells using MIB1 mouse mAb (green) and negative control (red).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q86YT6
Reactivity Human, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 2A7B1
Isotype Mouse IgG1
Calculated MW 110kDa
Description This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1). ;
Immunogen Purified recombinant fragment of human MIB1 (AA: 6-221) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application Note ELISA: 1/10000
WB: 1/500 - 1/2000
FCM: 1/200 - 1/400
Additional Information
Gene ID 57534
Other Names MIB; DIP1; ZZZ6; DIP-1; LVNC7; ZZANK2
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Monoclonal Antibody to MIB1 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MIB1
Synonyms DIP1, KIAA1323, ZZANK2
Function E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF- induced apoptosis (By similarity). Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation.
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite Cell membrane. Note=Localizes to the plasma membrane (By similarity). According to PubMed:15048887, it is mitochondrial, however such localization remains unclear Displaced from centriolar satellites in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock
Tissue Location Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined.
Research Areas
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References

1.J Cell Sci. 2015 May 1;128(9):1674-82. ; 2.Cell Res. 2012 Mar;22(3):603-6. ;

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$ 385.00
Cat# AO2430a
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