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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Mouse Monoclonal Antibody to MITF   

Mouse Monoclonal Antibody to MITF

Purified Mouse Monoclonal Antibody

     
  • E - Mouse Monoclonal Antibody to MITF AO2461a
    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • WB - Mouse Monoclonal Antibody to MITF AO2461a
    Western blot analysis using MITF mAb against human MITF (AA: 1-114) recombinant protein. (Expected MW is 38.9 kDa)
  • WB - Mouse Monoclonal Antibody to MITF AO2461a
    Western blot analysis using MITF mAb against HEK293 (1) and MITF (AA: 1-114)-hIgGFc transfected HEK293 (2) cell lysate.
  • FC - Mouse Monoclonal Antibody to MITF AO2461a
    Flow cytometric analysis of Hela cells using MITF mouse mAb (green) and negative control (red).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession O75030
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3A2E2
Isotype Mouse IgG1
Calculated MW 58.8kDa
Description This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.;
Immunogen Purified recombinant fragment of human MITF (AA: 1-114) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application Note ELISA: 1/10000
WB: 1/500 - 1/2000
FCM: 1/200 - 1/400
Additional Information
Gene ID 4286
Other Names MI; WS2; CMM8; WS2A; bHLHe32
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Monoclonal Antibody to MITF is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MITF
Synonyms BHLHE32
Function Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
Cellular Location Nucleus.
Tissue Location Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells. Isoform Mdel is widely expressed in melanocytes, melanoma cell lines and tissues, but almost undetectable in non-melanoma cell lines
Research Areas
Citations (0)

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References

1.Cell Mol Life Sci. 2015 Apr;72(7):1249-60.; 2.Int J Clin Exp Pathol. 2013 Jul 15;6(8):1658-64.;

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$ 385.00
Cat# AO2461a
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