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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Mouse Monoclonal Antibody to KRT10   

Mouse Monoclonal Antibody to KRT10

Purified Mouse Monoclonal Antibody

     
  • E - Mouse Monoclonal Antibody to KRT10 AO2462a
    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • WB - Mouse Monoclonal Antibody to KRT10 AO2462a
    Western blot analysis using KRT10 mAb against human KRT10 (AA: 345-454) recombinant protein. (Expected MW is 38.7 kDa)
  • WB - Mouse Monoclonal Antibody to KRT10 AO2462a
    Western blot analysis using KRT10 mAb against HEK293 (1) and KRT10 (AA: 345-454)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - Mouse Monoclonal Antibody to KRT10 AO2462a
    Western blot analysis using KRT10 mouse mAb against A431 (1), C6 (2), COS7 (3), Jurkat (4), NIH/3T3 (5), and HEK293 (6) cell lysate.
  • FC - Mouse Monoclonal Antibody to KRT10 AO2462a
    Flow cytometric analysis of Hela cells using KRT10 mouse mAb (green) and negative control (red).
  • IHC - Mouse Monoclonal Antibody to KRT10 AO2462a
    Immunohistochemical analysis of paraffin-embedded breast cancer tissues using KRT10 mouse mAb with DAB staining.
  • IHC - Mouse Monoclonal Antibody to KRT10 AO2462a
    Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using KRT10 mouse mAb with DAB staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, E
Primary Accession P13645
Reactivity Human, Mouse, Rat, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 3C2F5
Isotype Mouse IgG1
Calculated MW 58.8kDa
Description This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21.;
Immunogen Purified recombinant fragment of human KRT10 (AA: 345-454 ) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application Note ELISA: 1/10000
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
FCM: 1/200 - 1/400
Additional Information
Gene ID 3858
Other Names BIE; EHK; K10; KPP; BCIE; CK10
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Monoclonal Antibody to KRT10 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KRT10
Synonyms KPP
Function Plays a role in the establishment of the epidermal barrier on plantar skin.
Cellular Location Secreted, extracellular space. Note=Localized on the surface of desquamated nasal epithelial cells.
Tissue Location Seen in all suprabasal cell layers including stratum corneum
Research Areas
Citations (0)

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References

1.JAMA Dermatol. 2015 Jan;151(1):64-9. ; 2.Histopathology. 2012 Nov;61(5):910-20. ;

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$ 385.00
Cat# AO2462a
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