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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Mouse Monoclonal Antibody to TCF4   

Mouse Monoclonal Antibody to TCF4

Purified Mouse Monoclonal Antibody

     
  • E - Mouse Monoclonal Antibody to TCF4 AO2478a
    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • WB - Mouse Monoclonal Antibody to TCF4 AO2478a
    Western blot analysis using TCF4 mAb against human TCF4 (AA: 518-667) recombinant protein. (Expected MW is 43 kDa)
  • WB - Mouse Monoclonal Antibody to TCF4 AO2478a
    Western blot analysis using TCF4 mAb against HEK293 (1) and TCF4 (AA: 518-667)-hIgGFc transfected HEK293 (2) cell lysate.
  • FC - Mouse Monoclonal Antibody to TCF4 AO2478a
    Flow cytometric analysis of Hela cells using TCF4 mouse mAb (green) and negative control (red).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession P15884
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7B1B3
Isotype Mouse IgG2b
Calculated MW 71.3kDa
Description This gene encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome. In addition, an intronic CTG repeat normally numbering 10-37 repeat units can expand to >50 repeat units and cause Fuchs endothelial corneal dystrophy. Multiple alternatively spliced transcript variants that encode different proteins have been described. ;
Immunogen Purified recombinant fragment of human TCF4 (AA: 518-667) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application Note ELISA: 1/10000
WB: 1/500 - 1/2000
FCM: 1/200 - 1/400
Additional Information
Gene ID 6925
Other Names E2-2; ITF2; PTHS; SEF2; FECD3; ITF-2; SEF-2; TCF-4
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Monoclonal Antibody to TCF4 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TCF4
Synonyms BHLHB19, ITF2, SEF2
Function Transcription factor that binds to the immunoglobulin enhancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription (By similarity). Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00981, ECO:0000269|PubMed:22777675}
Tissue Location Expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. In developing embryonic tissues, expression mostly occurs in the brain
Research Areas
Citations (0)

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References

1.J Psychiatr Res. 2015 Oct;69:95-101. ; 2.Haematologica. 2014 Dec;99(12):e257-9.;

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$ 385.00
Cat# AO2478a
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