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> home > Products > Primary Antibodies > Antibody Collections > SARS CoV2 PPIs > Mouse Monoclonal Antibody to BRD2
Mouse Monoclonal Antibody to BRD2
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
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- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P25440 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 1H6B12 |
| Isotype | Mouse IgG1 |
| Calculated MW | 88kDa |
| Description | This gene encodes a transcriptional regulator that belongs to the BET (bromodomains and extra terminal domain) family of proteins. This protein associates with transcription complexes and with acetylated chromatin during mitosis, and it selectively binds to the acetylated lysine-12 residue of histone H4 via its two bromodomains. The gene maps to the major histocompatability complex (MHC) class II region on chromosome 6p21.3, but sequence comparison suggests that the protein is not involved in the immune response. This gene has been implicated in juvenile myoclonic epilepsy, a common form of epilepsy that becomes apparent in adolescence. Multiple alternatively spliced variants have been described for this gene.; |
| Immunogen | Purified recombinant fragment of human BRD2 (AA: 227-364) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Application Note | ELISA: 1/10000 WB: 1/500 - 1/2000 |
| Gene ID | 6046 |
|---|---|
| Other Names | FSH; NAT; RNF3; FSRG1; RING3; D6S113E; O27.1.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Mouse Monoclonal Antibody to BRD2 is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | BRD2 {ECO:0000303|PubMed:16227282, ECO:0000312|HGNC:HGNC:1103} |
|---|---|
| Function | Chromatin reader protein that specifically recognizes and binds histone H4 acetylated at 'Lys-5' and 'Lys-12' (H4K5ac and H4K12ac, respectively), thereby controlling gene expression and remodeling chromatin structures (PubMed:18406326, PubMed:17848202, PubMed:17148447, PubMed:20709061, PubMed:20048151, PubMed:20871596). Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation (PubMed:28262505). Plays a key role in genome compartmentalization via its association with CTCF and cohesin: recruited to chromatin by CTCF and promotes formation of topologically associating domains (TADs) via its ability to bind acetylated histones, contributing to CTCF boundary formation and enhancer insulation (PubMed:35410381). Also recognizes and binds acetylated non-histone proteins, such as STAT3 (PubMed:28262505). Involved in inflammatory response by regulating differentiation of naive CD4(+) T-cells into T- helper Th17: recognizes and binds STAT3 acetylated at 'Lys-87', promoting STAT3 recruitment to chromatin (PubMed:28262505). In addition to acetylated lysines, also recognizes and binds lysine residues on histones that are both methylated and acetylated on the same side chain to form N6-acetyl-N6-methyllysine (Kacme), an epigenetic mark of active chromatin associated with increased transcriptional initiation (PubMed:37731000). Specifically binds histone H4 acetyl-methylated at 'Lys-5' and 'Lys-12' (H4K5acme and H4K12acme, respectively) (PubMed:37731000). |
| Cellular Location | Nucleus. Chromosome Note=Detected on chromatin and nucleosomes |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
1.Mol Biol Cell. 2013 Nov;24(22):3557-68. ; 2.J Biol Chem. 2010 Mar 5;285(10):7610-8.;
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$ 385.00
Cat# AO2481a
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