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Mouse Monoclonal Antibody to BRD2

Purified Mouse Monoclonal Antibody

     
  • E - Mouse Monoclonal Antibody to BRD2 AO2482a
    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
    detail
  • WB - Mouse Monoclonal Antibody to BRD2 AO2482a
    Western blot analysis using BRD2 mAb against human BRD2 (AA: 227-364) recombinant protein. (Expected MW is 40.6 kDa)
    detail
  • WB - Mouse Monoclonal Antibody to BRD2 AO2482a
    Western blot analysis using BRD2 mAb against HEK293 (1) and BRD2 (AA: 227-364)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  • WB - Mouse Monoclonal Antibody to BRD2 AO2482a
    Western blot analysis using BRD2 mouse mAb against C6 (1) and Hela (2) cell lysate.
    detail
  • FC - Mouse Monoclonal Antibody to BRD2 AO2482a
    Flow cytometric analysis of Hela cells using BRD2 mouse mAb (green) and negative control (red).
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession P25440
Reactivity Human, Rat
Host Mouse
Clonality Monoclonal
Clone Names 7C1B10
Isotype Mouse IgG2b
Calculated MW 88kDa
Description This gene encodes a transcriptional regulator that belongs to the BET (bromodomains and extra terminal domain) family of proteins. This protein associates with transcription complexes and with acetylated chromatin during mitosis, and it selectively binds to the acetylated lysine-12 residue of histone H4 via its two bromodomains. The gene maps to the major histocompatability complex (MHC) class II region on chromosome 6p21.3, but sequence comparison suggests that the protein is not involved in the immune response. This gene has been implicated in juvenile myoclonic epilepsy, a common form of epilepsy that becomes apparent in adolescence. Multiple alternatively spliced variants have been described for this gene.;
Immunogen Purified recombinant fragment of human BRD2 (AA: 227-364) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application Note ELISA: 1/10000
WB: 1/500 - 1/2000
FCM: 1/200 - 1/400
Additional Information
Gene ID 6046
Other Names FSH; NAT; RNF3; FSRG1; RING3; D6S113E; O27.1.1
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Monoclonal Antibody to BRD2 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BRD2
Synonyms KIAA9001, RING3
Function May play a role in spermatogenesis or folliculogenesis (By similarity). Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling. Regulates transcription of the CCND1 gene. Plays a role in nucleosome assembly.
Cellular Location Nucleus. Note=Detected on chromatin and nucleosomes.
Research Areas
Citations (0)
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References

1.Mol Biol Cell. 2013 Nov;24(22):3557-68. ; 2.J Biol Chem. 2010 Mar 5;285(10):7610-8.;

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$ 385.00
Cat# AO2482a
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